Carbon monoxide (CO) causes the dysfunction of mitochondria to induce the apoptosis of cancer cells giving a promising choice as an emerging treatment. The currently reported CO-based complexes still suffer from many limitations. Synthesis of CO-release carriers in the manner of on-demand control is highly anticipated. In this study, we present a near-infrared (NIR) light-responsive CO-delivery nanocarrier, a PEGylated iron carbonyl derivatized Prussian blue (PB) nanoparticle (NP). Taking the structural characteristic containing Fe-N≡C-Fe unit, the -CN served as the active sites for the coordination of iron carbonyl, while the surface Fe sites chelated with the amine-functionalized polyethylene glycol (NH-PEG-NH) to yield PEGylated PB NPs carrying CO. The control of light intensity and exposure period is important to release the amount of CO as well as to deliver the hyperthermia effect. The combination therapy including CO and photothermal treatments displayed a synergistic effect against cancer cells. Importantly, the release of CO is inert in the blood circulation without NIR irradiation. The blood oxygen saturation measured by the pulse oximeter and the HCO, tCO, and pH values analyzed by the blood assay revealed the steady status from the mice studies, showing no acute CO poisoning.
A deficiency of nitric
oxide (NO) supply has been found to impair
wound healing. The exogenous topical delivery of NO is a promising
approach to enhance vasodilation and stimulate angiogenesis and collagen
deposition. In this study, the CN groups on the surface of Prussian
blue (PB) nanocubes were carefully reduced to −CH2–NH2 to conjugate with COOH group of hemin consisting
of a Fe-porphyrin structure with strong affinity toward NO. Accordingly,
the NO gas was able to coordinate to hemin-modified PB nanocubes.
The hemin-modified PB carrying NO (PB-NO) can be responsible to near-infrared
(NIR) light (808 nm) exposure to induce the thermo-induced liberation
of NO based on the light-to-heat transformation property of PB nanocubes.
The NO supply on the incisional wound sites can be readily topically
dropped the colloidal solution of PB-NO for receiving NIR light irradiation.
The enhanced blood flow was in a controllable manner whenever the
wound sites containing PB-NO received NIR light irradiation. The promotion
of blood perfusion following the on-demand multidelivery of NO has
effectively facilitated the process of wound closure to enhance angiogensis
and collagen deposition.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.