Actinobacillus actinomycetemcomitans, the etiologic agent of localized juvenile periodontitis, produces a potent leukotoxin that kills human neutrophils. The production of leukotoxin RNA can vary more than 50-fold among isolates of A. actinomycetemcomitans, and strains expressing high levels of leukotoxin RNA are most often found at sites of periodontal disease. To assess the relative contributions of transcription factors and promoter sequences in setting the disparate levels of leukotoxin RNA found, we have undertaken classical cis/trans analyses. First, the leukotoxin promoter regions from moderately leukotoxic (Y4) and minimally leukotoxic (ATCC 33384) strains of A. actinomycetemcomitans were cloned, sequenced, and compared with the previously sequenced leukotoxin promoter region of the high-producer strain JP2. The Y4 and ATCC 33384 promoter regions each contain a 528-bp segment that is absent from JP2. Interestingly, the analysis of various deletion constructs in A. actinomycetemcomitans indicated that Y4, despite the large insertion, initiates leukotoxin RNA synthesis at the same promoter as JP2 does. To perform cis/trans analyses, these three leukotoxin promoter regions were cloned into a plasmid upstream of the reporter gene -galactosidase. Each plasmid was transformed into JP2, Y4, and ATCC 33384, and the -galactosidase levels were determined. The results indicated that the sequences responsible for down-regulating leukotoxin RNA levels in Y4 relative to JP2 are found within the transcribed region of the Y4 leukotoxin operon. Importantly, in ATCC 33384, strain-specific trans factors and promoter sequence differences are equally significant in determining the lower levels of leukotoxin RNA. We hypothesize that either strain ATCC 33384 has a negative regulatory protein (which is missing or mutated in JP2/Y4) or that JP2 and Y4 carry an activator that is missing or mutated in ATCC 33384.
Aggregatibacter actinomycetemcomitans has been implicated as the primary etiologic agent in localized aggressive periodontitis. This bacterium produces a leukotoxin which may help the bacterium evade the host immune response. Leukotoxin transcription is induced when A. actinomycetemcomitans is grown anaerobically, as in the periodontal pocket. Previously, a 35 bp oxygen-response-element (ORE) was shown to be responsible for oxygen regulation at the leukotoxin promoter. However, the gene's transcription is not controlled by Fnr or ArcA, the major oxygen regulators in other bacteria. To identify the potentially novel protein(s) that regulate leukotoxin transcription, protein extracts of A. actinomycetemcomitans were tested for ORE binding by mobility shift assays; one ORE-specific binding complex was found. Standard fractionation protocols and protein sequencing identified the ORE binding protein as integration host factor (IHF). DNaseI protection assays showed that the IHF binding site overlaps the first half of the ORE. To assess the effect of IHF on leukotoxin synthesis, an A. actinomycetemcomitans deletion mutant in ihfB was constructed and characterized. Interestingly, leukotoxin RNA and protein synthesis was de-repressed in the ihf mutant, although leukotoxin synthesis in still oxygen-regulated in the mutant cells. Thus, IHF plays a direct role in repressing leukotoxin transcription, but another protein is also involved in regulating leukotoxin expression in response to oxygen.
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