Biological control of pests in agroecosystems could be enhanced by combining multiple natural enemies. However, this approach might also compromise the control efficacy through intraguild predation (IGP) among the natural enemies. Parasitoids may be able to avoid the risk of unidirectional IGP posed by entomopathogenic fungi through selective oviposition behavior during host foraging. Trybliographa rapae is a larval parasitoid of the cabbage root fly, Delia radicum. Here we evaluated the susceptibility of D. radicum and T. rapae to two species of generalist entomopathogenic fungi, Metarhizium brunneum isolate KVL 04-57 and Beauveria bassiana isolate KVL 03-90. Furthermore, T. rapae oviposition behavior was assessed in the presence of these entomopathogenic fungi either as infected hosts or as infective propagules in the environment. Both fungi were pathogenic to D. radicum larvae and T. rapae adults, but with variable virulence. When host patches were inoculated with M. brunneum conidia in a no-choice situation, more eggs were laid by T. rapae in hosts of those patches compared to control and B. bassiana treated patches. Females that later succumbed to mycosis from either fungus laid significantly more eggs than non-mycosed females, indicating that resources were allocated to increased oviposition due to perceived decreased life expectancy. When presented with a choice between healthy and fungal infected hosts, T. rapae females laid more eggs in healthy larvae than in M. brunneum infected larvae. This was less pronounced for B. bassiana. Based on our results we propose that T. rapae can perceive and react towards IGP risk posed by M. brunneum but not B. bassiana to the foraging female herself and her offspring. Thus, M. brunneum has the potential to be used for biological control against D. radicum with a limited risk to T. rapae populations.

Biological control of pest insects can be improved by providing natural enemies with additional food resources such as floral nectar within the production field. However, herbivores may also benefit from this practice. The aim of this 3‐year field study was to investigate if dill and buckwheat, aimed as food resources for natural enemies, could increase the densities of the cabbage root fly, Delia radicum L. (Diptera: Anthomyiidae), a severe pest on crucifers. Differences in egg density, numbers of pupae and sex ratio were compared between cabbage plots with or without flowers. Habitat manipulation by intercropping flowering plants with cabbage did not increase the overall D. radicum egg density in our 3‐year study, and there were no significant differences in egg numbers between treatments in any year. No effect on the fecundity of D. radicum was observed, most likely because of the high mobility and feeding behaviour of the female flies, combined with high abundance and diversity of other food sources around the fields during this period. Despite equal egg numbers, fewer pupae were found in plots with flowers than without in one of three studied years. This finding suggests that natural enemies attacking larvae and pupae of D. radicum were either more abundant or efficient in cabbage plots with flowers.

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To locate and evaluate host patches before oviposition, parasitoids of herbivorous insects utilize plant volatiles and host-derived cues, but also evaluate predator-derived infochemicals to reduce predation risks. When foraging in host habitats infested with entomopathogenic fungi that can infect both a parasitoid and its host, parasitoids may reduce the risk of intraguild predation (IGP) by avoiding such patches. In this study, we examined whether the presence of the entomopathogenic fungi Metarhizium brunneum and Beauveria bassiana in soil habitats of a root herbivore, Delia radicum, affects the behavior of Trybliographa rapae, a parasitoid of D. radicum. Olfactometer bioassays revealed that T. rapae avoided fungal infested host habitats and that this was dependent on fungal species and density. In particular, the parasitoid avoided habitats with high densities of the more virulent fungus, M. brunneum. In addition, host density was found to be important for the attraction of T. rapae. Volatiles collected from host habitats revealed different compound profiles depending on fungal presence and density, which could explain the behavior of T. rapae. We conclude that T. rapae females may use volatile compounds to locate high densities of prey, but also compounds related to fungal presence to reduce the risk of IGP towards themselves and their offspring.Electronic supplementary materialThe online version of this article (doi:10.1007/s00442-015-3326-2) contains supplementary material, which is available to authorized users.

This 3-year field study investigated the combined effect of floral resources and perennial shelter habitats (i.e. conservation strips), and crop rotation in supporting natural enemies of the cabbages root fly, Delia radicum. Habitat manipulation with conservation strips increased the overall catches of hymenopteran parasitoids. However, conservation strips did not increase parasitism by either of the two dominant parasitoid species, Trybliographa rapae and Aleochara bipustulata, in any study year. In fact, higher parasitism was found in control plots in the second year. This could be explained by parasitoid mobility and higher patch detectability, as more plants in the control plots were infested with D. radicum larvae. Conservation strips did not result in increased predation of D. radicum eggs. However, the activity densities of two Bembidion species were correlated with egg predation. The species assemblage distribution of epigeal predators was best explained by seasonal period, followed by year and, to a low extent, crop type, while treatment with conservation strips had no effect. However, during the egg laying peak of D. radicum, a higher number of A. bipustulata, an important larval predator was observed in conservation strips during one study year. In conclusion, positive effects of conservation strips were demonstrated for abundance of some natural enemies of D. radicum, but a consistent increase in performance could not be adequately demonstrated due to experimental set-up, the short timescale and the complex landscape in which our study site was located.