Sample diversion and bacterial culture are effective methods to reduce bacterial risk with WBP transfusion. Bacterial contamination of PSPs was assessed at 5.8-fold our current rate for apheresis PLTs utilizing comparable culture protocols.

In this study the authors examine the effects of common storage and handling procedures on serum alanine aminotransferase (ALT) levels. Clotted blood samples from 14 subjects were centrifuged at 280 X g for 10 minutes. Serum was tested immediately for baseline ALT levels and aliquots were stored at 22, 4, -20, and -80 degrees C. Additional serums were stored on the clot at 22 and 4 degrees C. ALT quantitation was performed at intervals of 3, 6, 9, and 24 hours and on Days 2, 4, 6, and 8. Unseparated tubes were sampled both proximal and distal to the red cells to determine ALT leakage from red cells lysis. For all storage conditions, gradual loss of activity occurred over time. Mean activity loss in separated serum at 2 days was 6 percent at 4 degrees C and 20 percent at 22 degrees C. Mean loss in unseparated serum was 3 percent at 4 degrees C and 12 percent at 22 degrees C. Marked ALT loss averaging 46 percent by day 6 occurred with storage at -20 degrees C, whereas storage at -80 degrees C resulted in an 8 percent decrease for the same period. For storage of up to 1 week, it is recommended that serum be separated early and retained at 4 degrees C. Frozen samples should be kept at -80 degrees C. Storage of separated or unseparated blood for up to 24 hours at 22 or 4 degrees C, as commonly found in blood centers, will marginally adversely affect the accuracy of ALT determination.

The brochures may have encouraged previous donors to return, but their use did not significantly increase the recruitment of new donors or the return of temporarily deferred donors.

A general-purpose laboratory robot was configured to prepare blood samples for the HBsAg and anti-HIV tests. Several issues were studied in order to eliminate problems and optimize the system. These included pipetting accuracy, intersample carryover, reagent interference, splashing and aerosol generation and user-related issues. A number of general guidelines were developed to assist others in developing and implementing systems for this and similar applications.

These studies indicate comparable in vitro and in vivo viability properties with 0- to 5-minute and 1-hour rest periods and at 4 hours (in vitro only).