What is the interplay of high-tech industrial agglomeration and urban innovation? How does high-tech industrial agglomeration affect urban innovation? What are the heterogeneous effects of high-tech industry agglomeration on urban innovation in different conditions? To answer these questions, this paper analyzes the interrelationship between high-tech industry agglomeration and urban innovation based on panel data of China’s Yangtze River Delta urban agglomeration from 2010 to 2019. We discuss the influence mechanism of high-tech industrial agglomeration on urban innovation by exploring the mediating effect of industrial structure optimization and the threshold effect of industrial attributes. The heterogeneous impact of high-tech industry agglomeration on urban innovation is also been further studied. We find that (1) the interaction relationship between high-tech industry agglomeration and urban innovation output is positive. (2) The advancement of industrial structure plays a positive intermediary role between high-tech industrial agglomeration and urban innovation output, while the rationalization of industrial structure shows a suppressing effect. (3) There are different threshold effects between capital intensity and technology intensity. The influence of high-tech agglomeration on urban innovation is positive only when the capital intensity exceeds 1.125. However, the influence is always positive in different levels of technology intensity, significantly. When the technology intensity is higher than 9.012E − 06, the degree and significance of this positive impact would decrease. (4) There are heterogeneous impacts of high-tech industry agglomeration on urban innovation output in the Yangtze River Delta urban agglomeration in different time stages, urban innovation development stages, and urban circles.
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