Background and aims: Currently sedation is a common practice in colonoscopy to reduce pain of patients and improve the operator satisfaction, while its impact on examination quality, especially polyp detection rate (PDR) and adenoma detection rate (ADR) is still controversial. Thus, we aimed to investigate the association of sedation with PDR/ADR. Methods: Consecutive patients receiving colonoscopy between January 2017 to January 2020 at the Nanjing Drum Tower Hospital were collected. Univariate and multivariate logistic regression models were performed to investigate the association between sedation and PDR/ADR. Subgroup analysis and propensity score matching analysis (PSM), as sensitivity analysis, were performed to validate the independent effect. Results: The PDR and ADR were significantly higher in cases with sedation (PDR: 55.4% vs 46.6%, OR: 1.42, 95% CI: 1.32~1.53, P < 0.001; ADR: 37.3% vs 30.2%, OR: 1.37, 95% CI: 1.27~1.49, P < 0.001). Multivariate analysis showed that the sedation was an independent factor associated with PDR (OR: 1.54, 95% CI: 1.40~1.69, P < 0.001) and ADR (OR: 1.45, 95% CI: 1.32~1.60, P < 0.001). The effect was consistent in subgroup analyses(P > 0.05) and PSM analysis (PDR: 56.1% vs 46.4%, OR: 1.48, 95% CI: 1.35~1.62, P < 0.001; ADR: 37.4% vs 30.0%, OR: 1.40, 95% CI: 1.27~1.54, P < 0.001). Conclusion: Sedation was associated with a higher polyp and adenoma detection rates during colonoscopy, which can promote the quality of colonoscopy.
Background. Assays of transposase accessible chromatin sequencing (ATAC-seq) is an efficient assay to investigate chromatin accessibility, which depends on the activity of a robust Tn5 transposase to fragment the genome while cutting in the sequencing adapters. Methods. We propose reliable approaches for purifying hyperactive Tn5 transposase by chitin magnetic bead sorting. Double-stranded DNA of J76 cells and 293T cells were digested and subjected to tagmentation as test samples with Tn5 transposase, and libraries were established and sequenced. Sequencing data was then analyzed for peak calling, GO enrichment, and motif analysis. Results. We report a set of rapid, efficient, and low-cost methods for ATAC-seq library construction and data analysis, through large-scale and rapid sequencing. These methods can provide a reference for the study of epigenetic regulation of gene expression.
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