In this study, the effects of oral administration of probiotic Bacillus sp. D2.2 and prebiotic from sweet potato extract on growth performance and resistance against Vibrio harveyi in Pacific white shrimp (Litopenaeus vannamei) were investigated. During 32-day feeding experiment, 360 individuals of Pacific white shrimp (PL15) with initial weight of 0.02 ± 0.002 g were fed with basal diet as control (A); supplemented with 6% probiotic and 0% prebiotic (B); 6% probiotic and 2% prebiotic (C); 6% probiotic and 4% prebiotic (D). At the end of feeding trial, weight gain (WG), average daily growth (ADG), feed conversion ratio (FCR), and survival rate (SR) were assessed. WG, ADG and FCR of the shrimp were significantly better in treatment D than those of the shrimp in other treatments. Control and treatment D as the best feeding trial were selected for challenge test with infectious V. harveyi. Survival rate and mean time to death (MTD) of the shrimp fed the supplemented diet were not significantly different (P>0.05) to the control. Infection levels in shrimp were evaluated using morphological scoring methods. Infection levels of V. harveyi in shrimp fed the diet were lower compared to the control.

Coconut waste is one of the industrial waste or household waste that potentially can be mixed in feed, because it contains of 5,7% protein, 16,3% fat, 38,1% carbohydrate, 31,6% rough fiber, 5,5% water, and 2,6% ash. The aim of this study was to learn the effectivity of adding shredded coconut waste as fish feed ingredients for dumbo catfish fry. This research was carried out in 60 days in Aquaculture laboratorium, Fisheries and Marine, Agriculture Faculty, University of Lampung. This research used completety randomized design with 4 treatments and 3 repetitions. Treatment A (0% of coconut waste flour), treatment B (10% of coconut waste flour), treatment C (20% of coconut waste flour), and treatment D (30% of coconut waste flour). The parameters in this research were growth rate, daily growth rate, and feed conversion ration and protein retention and water quality. The result showed that the use of coconut waste flour in feed gave no effect to the growth rate, daily growth rate, and feed conversion ratio for the dumbo catfish.

Soybean meal is one of the feed ingredients that is still imported, so indigofera leaf flour is tried as an alternative to local feed ingredients to reduce feed costs. The purpose of this study was to determine the effect of indigofera leaf flour (Indigofera sp.) as a substitute for soybean meal on growth performance on enlargement of striped catfish (Pangasianodon hypophthalmus). This study used a completely randomized design with 4 treatments and 3 replications. P1 (without substitution of indigofera leaf flour), P2 (substitution of soybean meal with 10% indigofera leaf flour), P3 (substitution of soybean meal with 20% indigofera leaf flour), P4 (substitution of soybean meal with 30% indigofera leaf flour). The data obtained were analyzed using analysis of variance (ANOVA) and further tested with Duncan's test. Based on statistical analysis, the test feeding had a significant effect (p<0.1) on absolute weight growth, specific growth rate, and feed efficiency and the yield was not significantly different (p>0.1) on absolute length and survival rate of fish. The results showed that the use of indigofera leaf flour as a substitute for soybean meal up to a limit of 20% was able to provide a relatively better growth performance than 30%.

Availability of good-quality shrimp feed is an important factor of shrimp increased production. High protein feed can be generated by adding raw materials that contains high protein. One of the raw materials that contains high protein is earthworm meal (Lumbricus sp.). This research goal was to study the influence of the substitution of fish meal by earthworm feed with different proportions toward growth and survival rate of tiger shrimp larvae (Penaeus monodon). The experimental design used Completely Randomized Design (RAL) consists of five treatments with three replications: Feed test A (25%  earthworm meal + 75% fish meal), feed test B (50% earthworm meal + 50% fish meal), feed test C (75% earthworms meal + 25% fish meal), feed test D (100% earthworms meal) and feed test E (commercial feed). Results showed that the tiger shrimp which given feed test E is the best result for tiger shrimp growth with 0.480 ± 0.076 grams/individual, while feed test B was the best result for tiger shrimp survival rate with 82 ± 3.46%., and on the feed test D was the best result for tiger shrimp feed conversion rate with 4.0 ± 0.17. Based on results of statistical tests showed that the substitution of fish meal with earthworm meal did not give significant effect on growth and survival rate of tiger shrimp.

Utilization of ex-sand pit lakes for aquaculture is difficult due to low water quality and high concentrations of iron (Fe). Phytoremediation using aquatic plants has been proven to be effective in reducing Fe in waters. This study aims to determine the growth, feed conversion efficiency and survival rate of tilapia (Oreochromis niloticus) cultured with ex-sand mining water media with phytoremediation treatment. Phytoremediation treatment was carried out by Eichhornnia crassipes, Azolla pinnata, and Salvinia molesta. Fish culture experiments were carried out in plastic tarpaulin tanks for 40 days, with ad satiation feeding, three times a day using the commercial feed. The results showed that phytoremediation with aquatic plants had succeeded in reducing Fe to a level suitable for fish culture. Fish culture experiments showed an absolute length growth rate of 0.09-0.18 cm/day and an absolute weight growth rate of 0.11-0.16 g/day. The feed conversion ratio was 1.18-1.40 and the survival rate was 98.04-99.08%. The survival rate of tilapia is high, the feed conversion ratio is medium and growth is low. The high environmental temperature and the decrease in water quality due to the absence of water changes and aeration are suspected of causing the low growth of fish. Therefore, further research with water change and aeration experiments and the use of other species of fish is needed to follow up the results of this study.

Gourami (Osphronemus goramy Lac.) is one of the freshwater fish commodities that has high economic value because of its delicious meat taste which is very popular with all people. However, the feed commonly used by carp cultivators is factory-made feed, namely commercial feed which usually costs quite a bit because some of the ingredients for commercial feed are imported from abroad. Therefore, it is necessary to find a solution to reduce the cost of aquaculture production by replacing commercial feed with homemade feed made from local raw materials. The purpose of this study was to examine the use of local raw materials formulated to make feed suitable for the growth of gourami and to analyze the cost of producing carp feed made from local raw materials. This study consisted of 4 treatments and 3 replications using a completely randomized design (CRD). The treatments consisted of treatment A (Protein 30%), B (Protein 33%), C (Protein 36%), and D (Commercial). The results of this study showed that the treatment of feed A, B, and C produced the same growth as commercial feed, and treatment of feed A resulted in the lowest cost of feed production compared to other treatments.

The research was aimed to know the growth and survival rate of mad barb fish which feed by maggot and pellet combination also the best proportion feed of maggot combination. The method in this research used completely randomized design (CRD) with 5 treatments i.e A (pellet 100%), B (pellet 75% and maggot 25%), C (pellet 50% and maggot 50%), D (pellet 25% and maggot 75%), E (maggot 100%).The result showed feed with maggot and pellet effected on growth and survival rate mad barb fish. The best treatment on growth rate (5,56 gram) and average daily grain (0,09 gram/day) is combination pellet 50% and maggot 50%. Where as the best treatment on feed conversion rate (1,69

Eel (Anguilla bicolor) is a export commodity fish in fisheries sector and has has high economic value but has slow growth. One method to accelerate eel growth is through feeding with the enrichment of fish oil. The aim of this research is to know the increase of growth rate of eel fish fed with the enrichment of essential fatty acids with fish oil. The study used 3 treatments and 3 replications ie A (0% fish oil / control), B (1.5% fish oil), and C (3% fish oil). Parameters observed included specific growth rate, feed consumption total, feed efiesiensi, fat retention, survival rate. The supporting parameters are water quality. The result of research obtained were tabulated and analyzed by excel 2013 program and SPSS v. 20.0. with 95% confidence interva and continued by Duncan test. The results showed that there was a significant effect (P <0.05) on the growth rate of eel fish fed with the enrichment of essential fatty acids with fish oil. Treatment C (3% fish oil) is the optimum dose because it has the best growth performance and best feed efficiency that is specific growth rate (4,61% ± 0,0075), total feed consumption (246,3 g ± 50,1), feed efiesiensi (42,5% ± 3,74), fat retention (2,97% ± 0,148) and survival rate (86,7% ± 11,5).