Calf brain tubulin was subjected to isoelectric focusing and tryptic peptide map analysis. Results from isoelectric focusing experiments showed a total number of 17 well-resolved protein peaks. The number of peaks and the mass distribution under each peak remained the same when the concentration of protein or ampholyte was altered. When the protein was subjected to two-dimensional isoelectric focusing, a diagonal pattern was observed, indicating that the multiple peaks observed are not a manifestation of tubulin- ampholyte interaction. Further investigation by isolating these individual subspecies and subjecting them to isoelectric focusing yielded single peaks corresponding to the original ones without generating the initial pattern of multiple peaks. Tryptic peptide maps showed that among the subspecies of the alpha subunit there are 26 spots that are common among them. There are, however, 7 +/- 1 spots that are unique in each subspecies. Similar observations were obtained for the subspecies of the beta subunit although there are only 2 +/- 1 unique spots in each subspecies. These results suggest that tubulin subunits probably consist of polypeptides with both constant and variable regions in their sequences. Identical results were obtained for canine and rabbit brain tubulin, indicating that tubulin polymorphism is common among brain tissues. Tubulin isolated by either the polymerization-depolymerization or the modified Weisenberg procedures yielded identical results. These results show that the same subspecies of tubulin are extracted by both isolation procedures.
The paper presents the result of qualitative and quantitative analyses of mycoflora in the air of Lucknow city and adjoining areas. Burkard slide sampler and Andersen two-stage volumetric samplers were employed for air sampling at forty places of Lucknow at different times of the year from January 1997 to February 1998. As many as thirty-five types of fungal spores and twenty-nine types of fungal colonies were registered. It has been envisaged that certain fungi such as Alternaria, Aspergillus, Chaetomium, Cladosporium, Curvularia, Diplodia, Epicoccum, Fusarium, Nigrospora, Penicillium. Rhizopus, Torula, Trichoderma, Trichothecium, etc., prevalent in the air are associated with the biodeterioration of cultural properties.
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