Backgound: In order to detect the resistance of Acinetobacter baumannii to β-lactam antibiotics in western Guilin, analyze the reasons for its resistance, and provide laboratory basis for clinical treatment; method for the β-lactamase gene of Acinetobacter baumannii, and to explore the relationship between the presence of the β-lactamase gene of Acinetobacter baumannii and multidrug resistance. Methods: From November 2020 to June 2022, 78 non-repeated Acinetobacter baumannii strains from the Second Affiliated Hospital of Guilin Medical University were collected, and their resistance to carbapenem antibiotics was analyzed. PCR was used to detect resistance-related genes (blaOXA-23, blaOXA-51, blaOXA-58, blaOXA-24, blaIMPand blaSIM) and virulence genes (cusE, abaI and bfmS). To analyze the relationship between drug resistance and virulence genes of Acinetobacter baumannii. Results: Most of the 165 non-enterobacteriaceae bacteria we collected from the hospital were Acinetobacter baumannii and Pseudomonas aeruginosa, and most of the specimen types were from sputum and secretions, and most of them were from respiratory and critical care diseases area and intensive care unit, among them, there are 78 strains of Acinetobacter baumannii 30 studied this time. After screening, 78 Acinetobacter baumannii strains were resistant to Cefazolin, Ampicillin, Amoxicillin/Clavulanate, Aztreonam, Chloramphenicol, Tetracycline, Cefotaxime, Ampicillin/Sulbactam, Piperacillin, Gentamicin, Ciprofloxacin, Levofloxacin, Moxifloxacin, Cefepime, Ceftazidime, Piperacillin/Tazobactam, Compound Sulfonamide, Meropenem, Imipenem, Amikacin, Polymyxin, resistance rates were 98.72%, 98.72%, 96.16%, 96.15%, 96.15%, 66.67%, 60.26%, 58.98%, 58.97%, 57.69%, 57.69%, 57.69%, 57.69%, 57.69%, 56.41%, 55.13%, 53.85%, 52.56%, 52.56%, 50%, 0%. Among them, 17 strains with drug resistance gene blaOXA-51 were detected, accounting for 21.79%; drug gene blaOXA-23, blaOXA-24, blaIMP, blaOXA-58, blaSIM gene were not detected; 17 strains with virulence gene bfmS was detected, accounting for 21.79%; abaI,csuE virulence gene was not detected. Conclusion: Acinetobacter baumannii in the hospital is highly resistant to carbapenems, mainly carrying blaOXA-51 resistance genes. Drug resistance is closely related to the virulence gene bfmS.
Purpose To better understand the distribution and drug resistance of Klebsiella pneumoniae and provide a basis for clinical treatment. Methods We studied 167 Klebsiella pneumoniae clinical specimens in the Second Affiliated Hospital of Guilin Medical University from November 2020 to June 2022. These clinical specimens were isolated from urine, blood, sputum, catheter, pus, drainage fluid, bile, joint fluid, alveolar lavage fluid, and wound/trauma/incision secretions were collected. The drug resistance of Klebsiella pneumoniae and clinical outcomes were analyzed. We designed primers based on relevant resistance genes in The Comprehensive Antibiotic Resistance Database, while a part of them also used primer sequences from the relevant literature. We detected the carbapenems gene using polymerase chain reaction (PCR) and sent the product to the company for sequencing and homology analysis. Results A total of 707 inpatients were recruited and 167 isolates of Klebsiella pneumoniae were obtained. 83 strains from sputum specimens (49.7%), 18 strains from wound/trauma/incision secretion specimens (10.8%), 15 strains from pus specimens (9.0%), and other strains from urine specimens, blood specimens, drainage fluid, bile specimens, bile specimens, catheter specimens, and joint fluid specimens (30.5%). Klebsiella pneumoniae has a high rate of resistance to ampicillin (AMP) (98.2%), tetracycline (TE) (36.5%), piperacillin (PIP) (36.5%), chloramphenicol (CHL) (35.3%), and cefazolin (CFZ) (33.5%). From the analysis of sequencing results, we found that 68 strains were missing membrane pore protein Ompk-35, 3 strains were detected carrying KPC-1/KPC-2 type, 12 strains were missing membrane pore protein Ompk-36, 62 strains were missing membrane pore protein Ompk-37, 1 strain was detected carrying NDM-1, and no VIM-1, IMP-4, OXA-48, SME-2. Conclusions Klebsiella pneumoniae mainly causes respiratory tract infections and has a high rate of resistance to cephalosporins, piperacillin, and other antibacterial drugs. The detection rate of missing membrane pore protein ompk-35/ompk-37 is high, and the more drug resistance gene species it carries, the more drug resistant drug species it is.
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