In conclusion, NF-κB mediates duodenal contractility disturbances, the generation of ROS and the increase in the expression of interleukin-6 and TNF-α induced by LPS. Sulfasalazine, PDTC and RO 106-9920 may be therapeutic drugs to reduce these effects.
Background: several diseases such as sepsis can affect the ileum. Lipopolysaccharide (LPS), an endotoxin present in the cell wall of gram negative bacteria, is a causative agent of sepsis.Objectives: the aims of this study were: a) to investigate the role of mitogen activated protein kinases (MAPKs) in the effect of LPS on the acetylcholine-induced contractions of rabbit ileum; and b) to study the localization of MAPKs in the ileum.Material and methods: ileal contractility was studied in an organ bath and MAPKs were localized by immunohistochemistry.Results: acetylcholine-induced contractions decreased with LPS. SB203580, SP600125 and U0126 blocked the effect of LPS on the acetylcholine-induced contractions. Phosphorylated p38 and ERK were detected in neurons of myenteric plexus and phosphorylated p38 and JNK in smooth muscle cells of ileum.Conclusion: we can suggest that p38, JNK, and ERK MAPKs are involved in the mechanism of action of LPS in the ileum. INTRODUCTIONGastrointestinal stasis is a common complication of sepsis (1,2). Lipopolysaccharide (LPS), an endotoxin present in the cell wall of gram negative bacteria, is widely used to reproduce sepsis symptoms. For example, LPS administration results in a decrease in contractility of ileal longitudinal smooth muscle (3).Mitogen activated protein kinases (MAPKs) are a family of protein kinases involved in many cellular programs such as cell proliferation, cell differentiation and cell death and it has been observed an abnormal activation of MAPKs in pathological circumstances such as sepsis. The most important groups of MAPKs are the p38, c-Jun-N-terminal kinase (JNK1/2) and extracellular signal-regulated kinase (ERK1/2) MAPK(4).The aims of this study were: (1) to investigate the role of MAPKs in the effect of LPS on the acetylcholine-induced contractions of rabbit ileum and (2) to study the localization of MAPKs in the ileum. MATERIALS AND METHODS Drugs and solutions Acetylcholine (ACh) and lipopolysaccharide (LPS, from AnimalsAll experimental protocols were approved by the Ethics Committee of the University of Zaragoza (Spain). Male New Zealand rabbits weighing 2-2.25 kg were kept with standard rabbit fodder and free access to water. Vol. 104. N.° 6, pp. 305-309, 2012 This work was presented, in part, at the "LXVII Congreso Mitogen activated protein kinases blockade improves lipopolysaccharide-induced ileal motor disturbances
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