Microelectrode arrays (MEAs) for stimulation and signal recording of in vitro cultured neurons are presented. Each MEA is composed of 60 independent electrodes with 59 working ones and one reference one. These electrodes are divided into 30 pairs. Through each pair of electrodes, four independent states can be realized to define the accessing modes of neurons cultured on the surface of the electrodes. A total MEA covers an area of 10 mm×10 mm. MEAs are fabricated in a silicon-based semiconductor process. An implemented MEA is bonded on a specially designed printed-circuit-board (PCB) and surrounded by a culture chamber. An impedance measurement has been made to verify the electrical characteristics of MEAs. The surface was modified to enhance the biocompatibility. A series of PC12 cells culture experiments validates the effectiveness of the modification. An extracellular signal recording experiment with acetylcholine (Ach) as a stimulant has been carried out, and the results show the feasibility of MEAs for extracellular action potential recording. Extracellular electrical stimulation and recording experiments have been carried out too. They indicate that MEAs can be used for extracellular stimulation, recording, simultaneous stimulation and recording, and isolation of PC12 cells network cultured in vitro.Keywords microelectrode arrays (MEAs), extracellular recording, stimulation and recording, neurons network, in vitro
CitationPan H X, LÜ X Y, Wang Z G, et al. Silicon-based microelectrode arrays for stimulation and signal recording of in vitro cultured neurons.
To study the nonlinear dynamic behavior of the bladed overhang rotor system with squeeze film damper (SFD), a blade-overhang rotor-SFD model is formulated using the lumped mass method and the Lagrange approach. The cavitated short bearing model is employed to describe the nonlinear oil force of the SFD. To reduce the scale of the nonlinear coupling system, a set of orthogonal transformations is employed to decouple the one nodal diameter equations of blades, which are coupled with the dynamical equations of the rotor, with other equations of blades. In this way, the original system with 16+4n (n≥3) degrees of freedom (DoF) is reduced to a system with 24 DoF only. Then the parametric excitation terms in the blade-overhang rotor-SFD model are simplified in terms of periodic transformations. The coupling equations are numerically solved and the solutions are used to analyze the dynamic behavior of the system in terms of the bifurcation diagram, whirl orbit, Poincaré map and spectrum plot. A variety of motion types are found such as multi-periodic, quasi-periodic, and chaotic motions. Moreover, the typical nonlinear dynamic evolutions including the periodic-doubling bifurcation and reverse bifurcation are noted. It is noticed that there exist apparent differences in the dynamic behavior between the blade-overhang rotor-SFD models without and with considering the effect of blades. bladed overhang rotor system, squeeze film damper, bifurcation, chaos
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