Folding flake-like CuO sub-microstructure was successfully prepared on a large scale using a facile 1, 6-hexanediamin assisted low-temperature solution approach. The growth mechanism of the sample was discussed based on the time-resolved experiments. The experimental results demonstrated that the microstructures of CuO strongly depended on the concentration of the 1, 6-hexanediamin. The as-prepared CuO sub-microstructures were dispersed in Chitosan solution to fabricate composite film on glass carbon electrode. The electrochemical study has shown that the folding flake-like CuO sub-microstructure exhibits highest catalytic effect on Hg 2? than other CuO sub-microstructures. The amperometric response exhibited that the folding flakelike CuO sub-microstructure modified glassy carbon electrode has a good response for Hg 2? with a linear range of 1-200 lM in pH 6.0 phosphate buffered solutions.
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