Histone deacetylases (HDACs) inhibitor is a promising new approach to the treatment of lung cancer therapy via inhibiting cell growth and inducing apoptosis. miR-15a and miR-16-1 are important tumor suppressors through modulating B cell lymphoma 2 (Bcl-2), Cyclin D1, D2, and others. However, whether HDACs inhibitor modulates the expression of miR-15a/16-1 in lung cancer is still unknown. The purpose of our study was to identify a new miRNA-mediated mechanism which plays an important role in the anti-cancer effects of HDACs inhibitor. We found HDACs inhibitors trichostatin A (TSA) and sodium butyrate upregulated the expression of miR-15a/16-1, residing in the host tumor suppressor Dleu2 gene, through increasing the histone acetylation in the region of Dleu2/miR-15a/16-1 promoter in lung cancer cells. Moreover, among class Ι HDACs subtypes, only knockdown of HDAC3 by specific siRNA increased the hyperacetylation of Dleu2/miR-15a/16-1 promoter region and finally resulted in the upregulation of miR-15a/16-1. Furthermore, overexpression of miR-15a/16-1, which were always deleted or downregulated in lung cancer cells, effectively suppressed cell growth and reduced colony formation. Finally, TSA reduced the expression of Bcl-2, an important survival protein in lung cancer cells, partly through upregulation of miR-15a/16-1. Therefore, this offers a therapeutic strategy that lung cancer patients who exhibit low level of miR-15a/16-1 or high activity of HDACs may benefit from HDACs inhibitor-based therapy.
Nanosilver was modified by aptamer (ssDNA) to obtain a resonance scattering (RS) probe (AgssDNA) for melamine (MA). Based on the catalytic effect of the probe on the Fehling particle reaction, a nanocatalytic RS assay is proposed for the determination of 0.02-1.06 μg L(-1) MA.
The aptamer (ssDNA) was used to label nanogold (NG) particle to fabricate an aptamer-nanogold (NGssDNA) probe for melamine. The probe was stabile in pH 6.6 Na(2)HPO(4)-NaH(2)PO(4) buffer solutions and in the presence of high concentration of electrolyte. Upon addition of melamine, it interacted with the probe to form big NGssDNA-melamine aggregations that led to the resonance Rayleigh scattering (RRS) intensity at 566 nm increased greatly. The increased RRS intensity (ΔI) is linear to melamine concentration in the range of 1.89-81.98 μg/L, with a detection limit of 0.98 μg/L melamine. The unreacted probe in the aptamer reaction solution exhibited strong catalytic effect on the slow Cu(2)O particle reaction between glucose and Fehling reagent, but the catalytic activity of NG aggregations is very weak. When melamine concentration increased, the unreacted probe decreased, the RRS peak intensity at 614 nm decreased. The decreased RS intensity is linear to melamine concentration in the range of 0.63-47.30 ng/L melamine, with a detection limit of 0.38 ng/L. The aptamer-modified nanogold catalytic RRS assay was applied to determination of melamine in milk, with high sensitivity and selectivity, simplicity and rapidity.
The small nanosilver was prepared by the sodium borohydride procedure. The aptamer was used to modify nanosilver to obtain a nanosilver‐aptamer (AgssDNA) SERS probe for the determination of melamine. In pH 6.6 phosphate buffer solution and in the presence of NaCl, the AgssDNA probe specifically combined with melamine to release nanosilver particles that were aggregated to nanosilver clusters, which exhibited SERS effect at 240 cm−1. When melamine concentration increased, the nanosilver clusters increased, and the SERS intensity at 240 cm−1 increased. The increased SERS intensity ΔI240 cm−1 is linear to melamine concentration in the range of 6.3–403.6 μg·L−1, with a detection limit of 1.2 μg·L−1. This assay was applied to determination of melamine in milk, with satisfactory results.
Nanosilver of 10-nm size was prepared by the NaBH 4 -sodium citrate procedure, and it was modified by a single-strand DNA (ssDNA) aptamer to fabricate an AgssDNA probe for melamine. The probe was stabile at pH 7.0 Na 2 HPO 4 -NaH 2 PO 4 buffer solutions and in the presence of 25.0 mmol/L NaCl. Upon the addition of melamine, it interacted with the probe to aggregate big clusters, which led to the resonance scattering (RS) intensity at 470 nm increasing greatly. Under the selected conditions, the increased RS intensity (ΔI 470 nm ) is linear to melamine concentration in the range of 6.31-378.4 μg/L, with a regression equation of $I 470nm ¼ 1:124c þ 10:8 and a detection limit of 3.1 μg/L. The aptamer-modified nanosilver RS assay has been applied for the determination of melamine in milk, with satisfactory results.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.