Background
The primary aim of this study was to observe the effect of 5-ALA-mediated photodynamic therapy on oral squamous cell carcinoma in vitro.
Methods
SCC25 cells were divided into the observation group and the blank control group. Different concentrations of 5-ALA and SCC25 cells were co-incubated for different times, and the concentration of protoporphyrin IX was detected by flow cytometry. SCC25 cells were divided into the 5-ALA group (100 mg/L), the laser irradiation group alone, the 5-ALA plus laser irradiation group, and the blank control group (0 mg/L 5-ALA), and the methyl thiazolyl tetrazolium (MTT) solution method was used (each group was incubated for 4, 8 and 12 h in turn). The cell survival rate was calculated. Using annexin V-fluorescein isothiocyanate/propidium iodide method, the apoptosis of SCC25 cells was detected by flow cytometry.
Results
The level of protoporphyrin IX in SCC25 cells increased with increased concentrations of 5-ALA and length of incubation. However, after 12 h, protoporphyrin IX level in SCC25 cells was gradually stabilized, and similar effect was obtained with 100 mg/L or more 5-ALA, indicating that the level of protoporphyrin IX in SCC25 cells was determined by 5-ALA concentration and incubation time. 5-ALA plus laser irradiation exerted an inhibitory effect on the growth of SCC25 cells, which was highly associated with drug dose and incubation time. Compared with the control group, laser irradiation alone or 5-ALA alone had no effect on the apoptosis of SCC25 cells. Different concentrations of 5-ALA combined with laser irradiation showed a remarkable effect of apoptosis, and a higher apoptosis rate was seen with higher drug concentrations.
Conclusion
5-ALA-mediated photodynamic therapy affects the growth of SCC25 cells in vitro, which may provide a new idea for the clinical treatment of oral squamous cell carcinoma.
Cisplatin, a non-specific cell cycle antineoplastic drug, has therapeutic effects on a variety of malignant tumors. However, cisplatin has some drawbacks, such as poor targeting, which can lead to damage in normal tissue adjacent to a cancer, causing serious side effects in patients.
In this study, we designed and developed a folic acid (FA)-mediated cisplatin magnetic nanodrug, FA-CDDP-MNPs, and investigated its efficacy in the treatment of oral squamous cell carcinoma (OSCC). In vitro and in vivo experiments showed that FA-CDDP-MNPs had a greater inhibitory
effect on OSCC than CDDP alone. Due to the introduction of FA, the targeting of FA-CDDPMNPs was improved, and its cytotoxicity reduced. The successful design and development of FA-CDDP-MNPs provides a basis for novel drug development and design in the future.
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