Novel molecular markers are required for defining subsets of diffuse astrocytic tumor patients with differing prognoses. Here, we examined ATP2A2 expression in 109 human diffuse astrocytic tumor samples (39 grade II diffuse astrocytoma (DA), 19 grade III anaplastic astrocytoma (AA), 51 grade IV glioblastoma) and its correlation with patient clinicopathologic characteristics. ATP2A2 expression significantly correlated with tumor grade and survival (P<0.05). High ATP2A2 expression was detected in 35.3% (18/51) of glioblastoma patients, compared to 61.5% (24/39) in grade II, and 52.6% (10/19) in grade III astrocytoma patients (P=0.043). The median survival was 45±5.3 (95% CI, 34.7-55.3) months in patients with high ATP2A2 expression and 16±5.0 (95% CI, 6.3-25.7) months in patients with low ATP2A2 expression (P<0.0001). Additionally, high grade astrocytoma patients with high ATP2A2 expression showed longer survival (median, 31.0±4.9 months, 95% CI, 21.4-40.7) than those with low ATP2A2 expression (median: 13.0±1.6 months, 95% CI, 9.9-16.1; P=0.027). Furthermore, both ATP2A2 overexpression and IDH1 mutation were detected in secondary glioblastoma, AA developed from DA and oligodendrogiomas with IDH1 mutation. The MTT assays showed that lentiviral ATP2A2 overexpression significantly suppressed the clonogenic growth of glioblastoma U251MG cells (P<0.05). Xenografts stably overexpressing ATP2A2 were markedly smaller in size 4 weeks post inoculation (P<0.05). Our findings identified high ATP2A2 expression in a subset of astrocytoma patients that was associated with better prognosis and ATP2A2 suppressed astrocytoma growth.
We present here a case report of a seminal vesicle cyst (SVC) in a young man with epididymal tissue in a dysgenetic kidney and ectopic ureteral insertion. SVCs are usually found in the second to fourth decades of life. SVCs can be congenital or acquired. Congenital SVCs seem to be invariably associated with renal dysplasia, hypoplasia, agenesis and duplication abnormalities in the same side, with the exception of two cases [1,2].We report here a case report of a 26-year-old man who was referred to our hospital owing to absence of the left kidney, identified by sonography during a health examination. Magnetic resonance imaging (MRI) and magnetic resonance urography (MRU) showed a cyst expanding from the kidney fossa to the seminal vesicle fossa instead of the left kidney, ureter and seminal vesicle (Figure 1). His right urinary tract was normal. A solid mass was present in the left side of the bladder, and the left ureteral orifice could not be seen with a cystoscope. Laboratory tests of the blood and urine were normal. Clinical examination showed that the patient had a normal pair of testes, epididymis and penis.The surgeons performed a retroperitoneoscopy together with a small inclined inferior abdominal incision under general anaesthesia. At first, the patient was recumbent on his right arm. The cyst was resected in the postperitoneal region. There were three laparoscopic ports: a 12-mm trocar port at the left postaxillary line below the 12th rib, a 5-mm trocar port at the preaxillary line and 2 cm above the spina iliaca and a 10-mm trocar port at the preaxillary line below the arcus costarum. During the operation, the surgeons noticed that the cyst was just as shown by MRI and MRU. There were no vessels to the left kidney. The cyst was dissociated easily. The ectopic ureter opened in the seminal vesicle cyst, which was filled with chyliform fluid. At first, the surgeons suspected that the fluid was liquor puris, but it was later found to be semen with few sperm, which had poor vitality. Thereafter, the patient was moved to the Trendelenburg position. A small inclined inferior abdominal incision was made at the left lateral border of the rectus muscle. This cyst was resected completely.The total operating time was 120 min, with an estimated blood loss of 100 mL. The patient was able
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