Protein acetylation, regulated by acetyltransferases and deacetylases, is an important post-translational modification that is involved in numerous physiological and pathological changes in peripheral nerves. There is still no systematical analysis on the expression changes of protein acetylation regulators during sciatic nerve development, injury, and regeneration. Here, we sequenced and analyzed the transcriptome of mouse sciatic nerves during development and after injury. We found that the changes in the expression of most regulators followed the rule that “development is consistent with regeneration and opposite to injury.” Immunoblotting with pan-acetylated antibodies also revealed that development and regeneration are a process of increased acetylation, while injury is a process of decreased acetylation. Moreover, we used bioinformatics methods to analyze the possible downstream molecules of two key regulators, histone deacetylase 1 (Hdac1) and lysine acetyltransferase 2b (Kat2b), and found that they were associated with many genes that regulate the cell cycle. Our findings provide an insight into the association of sciatic nerve development, injury, and regeneration from the perspective of protein acetylation.
As one of the disadvantaged groups in society, the disabled should be helped to protect their rights and interests. However, there are still many difficulties in the economy, education and interpersonal relationship of the disabled in China, such as low income, difficult employment, large medical expenses, insufficient allocation of public resources and travel obstacles. The causes of these difficulties lie in the multiple social exclusion factors such as economic exclusion, institutional exclusion and relational exclusion, which hinder the reasonable rights and interests of the disabled. Therefore, we should persist in advocating the spirit of humanitarianism, build a multi-support platform, and give full play to the initiative of the disabled, so as to establish a support path that meets the real demands of the disabled in China.
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