Protein crystallization is affected by many parameters, among which certain parameters have not been well controlled. The temperature at which the protein and precipitant solutions are mixed (i.e., the ambient temperature during mixing) is such a parameter that is typically not well controlled and is often ignored. In this paper, we show that this temperature can influence protein crystallization. The experimental results showed that both higher and lower mixing temperatures can enhance the success of crystallization, which follows a parabolic curve with an increasing ambient temperature. This work illustrates that the crystallization solution preparation temperature is also an important parameter for protein crystallization. Uncontrolled or poorly controlled room temperature may yield poor reproducibility in protein crystallization.
Film cooling technology is developed to enhance the temperature resistant of nickel-base single crystal alloy blade. The shape, dimension, and arrangement of cooling holes impact the blade strength and life grievously. In this paper, the influences of holes arrangement on creep characteristic of cooling holes in the plate sample are investigated. The constitutive model for creep considering both cavitation and degradation damage is developed to predict the creep behavior of cooling holes. Results show that there are stress interferences among cooling holes. The distance and radius of the cooling holes impact the creep behavior of cooling holes seriously. Decreasing horizontal distance of the holes results in creep time reducing. On the contrary, increasing the vertical distance of the holes makes the creep time reduced.
Film cooling as an important thermal protection technology is widely used in aviation and ground gas turbine blades. But film cooling holes reduce the strength of blade seriously, which have become a key region of crack nucleation. In this paper, the plastic behaviors of nickel‐base single crystal alloy turbine cooling holes in spanwise injection angles range from 0° to 40° are investigated on basis of crystallographic constitutive theory. The results show that there are both higher stress regions and lower stress regions around multi‐column cooling holes, where suffer stress interference. The maximum Mises stress occurs at the hole in the center column. The places where the maximum resolved shear stresses occurs change with load and spanwise injection angle. The maximum Mises stress around holes with injection angle of 0° is lowest. With the injection angle increases, the maximum Mises stress increases until injection angles up to 30°. In all the slip systems, the resolved shear stress of hexahedral slip system is most sensitive to the changing of spanwise injection angle and load.
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