Abstract. Rahmi Z, Yurnaliza Y, Hastuti LDS. 2022. Isolation and screening of lovastatin-producing endophytic fungi from lemongrass (Cymbopogon nardus L.). Biodiversitas 23: 4189-4194. Fungi have long been studied for their cholesterol-lowering compounds, known as statins and the derivatives through fermentation in laboratory investigations. Therefore, this study aimed to isolate and screen endophytic fungal strains from lemongrass capable of producing lovastatin. Endophytic fungi were isolated from the stems and leaves of lemongrass (Cymbopogon nardus) and the selection of their ability to produce lovastatin was assayed using three approaches, namely negative test against Candida albicans, measurement based on absorbance at a wavelength (?) of 230 nm or colorimetric assay, and observation using thin layer chromatography (TLC). A total of 22 endophytic fungi, 15 from the leaves and 7 from the stems were isolated from lemongrass. The capability to produce lovastatin was confirmed using three approaches, resulting in three potential fungal strains, namely Trichoderma sp. CNB 2.5.3, unidentified fungus CND 2.5.4, and Nigrospora sp. CND 2.1.1. Based on the colorimetric assay, the potential strains produced a significant zone of inhibition against C. albicans, with greater lovastatin levels than the other isolates. The TLC analysis showed that the fungal ethanolic extracts contained crude lovastatin as indicated from the same retention factor (Rf) value as standard lovastatin. The presence of lovastatin-producing endophytic fungi can be investigated progressively for optimum production through fermentation and evaluation of its cholesterol-lowering activity in the future.
Root-knot nematodes are harmful organisms which cause severe damage to plants and decreasing its productivity. Biocontrol agents isolated from potential environment may solve the intriguing problem of these nematodes. Nematophagous fungi has proven as promising strategy in controlling nematodes when compared to commercial pesticides which may pollute the soil and environment through accumulated residues post-usage. Nematophagous fungi were isolated from several soils or sediments around Lau Kawar Lake using a combination of pour and sprinkle techniques. Nine fungal morphotypes were recovered from soil/sediment samples as revealed from their distinctive morphologies. Two isolates, LK.10.1 and LK.10.3 were designated as potential nematophagous fungal isolates by their effectiveness in killing nematodes within 36 hr of observation. Species-level identification is currently carried out for the isolates to reveal and confirm their identities as nematophagous fungi.
Root-knot nematodes are important agricultural pests causing serious economical loss to harvestable crops. Biological control using nematophagous fungi is one option to mitigate these infection through mechanism of physical or chemical killing methods. The present study tried to explore a possibility of finding native nematophagous fungal strains in the hope on discovery of novel and potential isolates originating from freshwater region of Toba Lake, North Sumatra, Indonesia. Isolation of nematophagous fungi was based on sprinkle and pour combination method on Chloramphenicol-Water Agar (CWA) incubated for 30 days. Freshwater samples of soil and sediments were collected from 28 sampling sites characterized by different anthropogenic activities as natural, fishery, residential, plantation and tourism area. Daily examination is conducted to obtain a single culture of nematophagous fungi sub-cultured on Potato Dextrose Agar (PDA) medium. Eleven isolates were observed to initiate predatory activities against tested Caenorhabditis elegans based on qualitative screening. The isolates showed either mechanical killing or chemical killing of nematodes during co-inoculation with nematodes. Micromorphological and molecular analysis are currently being conducted to obtain species identity from each isolate.
Kabupaten Deli Serdang merupakan kabupaten yang sudah lama terbentuk pada tahun 1946. Kabupaten Deli Serdang memiliki potensi pengembangan pertanian yang sangat besar seperti sub sektor tanaman pangan dan sub sektor tanaman hortikultura, serta daerah wisata lainnya.Kecamatan yang memiliki potensi pengembangan pengolahan salak lanjutan adalah Sinembah Tanjung Muda Hulu. Desa ini memiliki potensi usaha pertanian dan wisata alam. Masyarakat desa kurang lebih 30% warga yang tinggal di desa ini memiliki lahan untuk di tanami bibit salak yang mereka ambil dari Yogyakarta. Metode pelaksanaan kegiatan menjelaskan tahapan atau langkah-langkah dalam melaksanakan solusi yang ditawarkan untuk mengatasi permasalahan yang memuat: Permasalahan dalam aspek produksi. Metode pelaksanaan kegiatan menjelaskan tahapan atau langkah-langkah dalam melaksanakan solusi yang ditawarkan untuk mengatasi permasalahan yang memuat: Permasalahan dalam aspek produksi dan Permasalahan dalam aspek manajemen dengan diskusi dan ceramah. Tujuan program desa binaan ini adalah mengenalkan dan menerapkan strategi yang tepat dalam mengembangkan usaha dan pengolahan buah salak lanjutan sehingga dapat meningkatkan produksi salak lanjutan yang sudah diolah serta meningkatkan pendapatan masyarakat, khususnya petani
Three specimens of Cyathus spp. were collected during a field survey conducted in Tongkoh village and Taman Hutan Raya forest, North Sumatera. The three specimens were preliminary identified leading to one species, namely Cyathus montagnei. A description of North Sumatran C.striatus is given. From our knowing, this is the first report of Cyathus montagnei collected in North Sumatera with locality of Karo regency. Hence, further exploration upon identified species and remaining unexplored species is needed to reveal their assemblages or species richness in North Sumatera.
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