Endosperms from seeds of different subfamilies of Leguminosae were submitted to sequential aqueous and alkaline aqueous extractions. The extractions from species belonging to the Mimosoideae and Faboideae subfamilies yielded galactomannans with constant Man:Gal ratios, whereas the extractions from Caesalpinioideae seeds gave rise to galactomannans with increasing values of the Man:Gal ratio. The presence of a family of galactomannans within the same species may be a trait found only in Caesalpinioideae subfamily. The final insoluble residues that were obtained after the removal of galactomannans from the Caesalpinioideae and Faboideae subfamilies are composed of pure mannans and do not contain cellulose, while those from the Mimosoideae subfamily are composed of cellulose. A mannan was isolated from the unripe endosperm of Caesalpinia pulcherrima, suggesting no developmental relationship between galactomannan and mannan. These results are consistent with the presence of a distinctive cell wall pattern in the endosperms of Leguminosae species.
RESUMO -A comparação da metabolização da lectina de Cana valia hrasiliensis com as demais proteínas de reservas durante a germinação das sementes na presença de luz foi acompanhada através da combinação de dosagem de proteínas solú-veis, determinação da atividade hemaglutinante, cromatografia de afinidade em coluna de Sephadex G-50 e eletroforese em gel de poliacrilamida em presença de dodecilsulfato de sódio (SDS) e 2-mercaptoetanol. Os resultados foram comparados com resultados anteriormente obtidos com sementes germinadas na ausência da luz e ficou evidenciado que, mesmo com as plântulas fotossintetizando , a lectina de Canavalia brasiliensis é preservada, sendo metabolizada após as, demais proteínas de reserva. Estes dados sugerem também que estas proteínas devam ter funções específicas durante o ciclo vital da planta e não se constituem apenas em proteín5~s de reserva. 'Palavras-chave: Lectinas, Canavalia brasiliensis, germinação.ABSTRACT -Canavalia brasiliensis seed lectin during germination in the presence of light. The mobilization of the lectin of Canavalia brasiliensis during se~d germination, in the presence of light, was studied in comparison with the reserve proteins. Methods employed were determination of soluble protein con tent, hemagglutinating acti vi ty , affinity chromatography on Sephadex G-50 column , and PAGE-SDS. The results showed that the lectin was metabolized later than the reserve proteins suggesting that it may have a special function other than a simple reserve protein.
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