This study was performed to evaluate the health information system regarding the dengue surveillance system in Indonesia. Major obstacles to the implementation of an effective health information system regarding dengue cases in Bandung are examined, and practical suggestions on measures to overcome them are discussed. The study utilized a mixed-method research design using qualitative approaches: document analysis, key informants and focus group interviews. Thirty key informants were selected, comprised of policymakers, senior managers, and staff at the Ministry of Health. Data from documents and transcripts were evaluated through a modified Institutional Analysis and Development (IAD) framework described by Ostrom. Through this study, we have identified several issues that hinder the effective implementation of the health information system in the case of dengue in Bandung. In the end, we propose several recommendations for reform that encompasses motivational, strategic, and structural approaches to each component of the analysis. Through evaluation of the health information system for dengue surveillance in Indonesia, we conclude that well-coordination in multi-level governance in a country as large as Indonesia is the key in the implementation of the health information system in different levels of agencies. Furthermore, the adaptability of human resources in adopting a new information system also plays an important part.

Introduction: We aim to describe the performance of combined IgM and IgG point-of-care antibody test (POC-Ab) (Wondfo®) compared to real-time reverse transcriptase (rRT-PCR) (Allplex™ 2019-nCoV Assay) in detecting coronavirus disease 2019 (COVID-19). Methodology: We compared POC-Ab with rRT-PCR results among patients in a tertiary hospital from January to March 2020 in Bandung, Indonesia. We selected presumptive COVID-19 patients with positive rRT-PCR consecutively and 20 patients with negative rRT-PCR results were selected randomly from the same group of patients as controls. We described the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) with corresponding 95% confidence interval using serum and capillary blood samples. We also tested POC-Ab using non-COVID-19 (confirmed dengue and typhoid) patients’ sera. Results: Twenty-seven patients with positive rRT-PCR result and 20 negative controls were included (68.1% males, mean age 46 (SD: 15.4)). Using the serum, the sensitivity of the POC-Ab was 63.0% (42.4-80.6), specificity was 95.0% (75.1-99.9), PPV was 94.4% (72.7-99.8), NPV was 65.5% (45.7-82.1). A subset of 20 patients was tested using a capillary blood sample. The accuracy of the capillary blood sample is lower compared to serum (50.0% vs. 78.7%). None of the non-COVID-19 sera tested were reactive. Conclusions: POC-Ab for COVID-19 has a high specificity with no false-positive result in non-COVID-19 sera. Therefore, it can be used to guide diagnostic among symptomatic patients in resource limited settings. Given its low sensitivity, patients with high suspicion of COVID-19 but non-reactive result should be prioritized for rRT-PCR testing.

A single‐chain variable fragment (scFv) is an antibody fragment composed of VH and VL linked by a hydrophilic linker that can be designed according to the shape of the target molecule and synthesized in prokaryotic or eukaryotic cells via biotechnology engineering. This study developed an electrochemical immunosensor that detects the RBD of SARS‐CoV‐2 using a screen‐printed carbon electrode modified with gold nanoparticles, and scFv as a bioreceptor. Electrochemical impedance spectroscopy was employed to measure specific interactions of antigens with antibodies. The developed immunosensor had a limit of detection and a quantification limit of 4.86 ng mL−1 and 16.20 ng mL−1, respectively. The immunosensor was stable at room temperature for up to 30 days’ storage. The immunosensor was assessed at biosafety level 3 using 33 nasopharyngeal swab specimens (clinical samples); the pieces of data were compared with quantitative Reverse Transcriptase‐PCR. The agreement of the data, the low detection limit achieved, the rapid analysis (30 min), the miniaturization, and the portability of the instrument combined with the easiness to use has the potential to become Point of Care (POC) for diagnosing the COVID‐19 disease.

Dengue Hemorrhagic Fever (DHF) is a major mosquito-borne viral disease. Studies have reported a strong correlation between weather, the abundance of Aedes aegypti, the vector of DHF virus, and dengue incidence. However, this conclusion has been based on the general climate pattern of wide regions. In general, however, the human population, level of infrastructure, and land-use change in rural and urban areas often produce localized climate patterns that may influence the interaction between climate, vector abundance, and dengue incidence. Thoroughly understanding this correlation will allow the development of a customized and precise local early warning system. To achieve this purpose, we conducted a cohort study, during January-December 2017, in 16 districts in Bandung, West Java, Indonesia. In the selected areas, local weather stations and modified light mosquito traps were set up to obtain data regarding daily weather and the abundance of adult female Ae. aegypti. A generalized linear model was applied to analyze the effect of local weather and female adult Ae. aegypti on the number of dengue cases. The result showed a significant non-linear correlation among mosquito abundance, maximum temperature, and dengue cases. Using our model, the data showed that the addition of a single adult Ae. aegypti mosquito increased the risk of dengue infection by 1.8%, while increasing the maximum temperature by one degree decreased the risk by 17%. This finding suggests specific actionable insights needed to supplement existing mosquito eradication programs.

Pada tahun 2014, Bandung memiliki angka kasus demam dengue (DD) tertinggi dari 27 kabupaten/kota di Jawa Barat. Upaya pengendalian DD telah dilaksanakan sejak beberapa dekade yang lalu. Salah satu upaya adalah dengan mengeliminasi tempat perkembangbiakan nyamuk melalui peran serta masyarakat. Penelitian ini bertujuan menilai partisipasi masyarakat Kota Bandung dengan mengetahui tempat-tempat perkembangbiakan Aedes aegypti baik di dalam maupun di luar rumah. Sampling dilakukan di 16 kelurahan di Kota Bandung yang telah dipilih berdasar atas angka kejadian DD, kepadatan penduduk, ketinggian permukaan, dan status sosial-ekonomi periode 2015. Populasi penelitian adalah rumah-rumah yang terdapat di 16 kelurahan tersebut. Jumlah sampel penelitian ini adalah 1.983 rumah yang merupakan perwakilan dari tiap kelurahan. Sampling jentik dilakukan pada berbagai tempat penampungan air, baik penampungan alami maupun buatan di sekitar pemukiman penduduk. Jentik yang ditemukan dimasukkan ke dalam wadah dan dibawa ke laboratorium untuk diidentifikasi dan dihitung jumlahnya. Hasil menunjukkan tempat perkembangbiakan nyamuk yang paling dominan adalah bak mandi (50%), talang air (24%), dan dispenser (15%). Data entomologi diperoleh hasil House index (HI) 24%, Container index (CI) 12%, dan Breteau index (BI) 36%. Hasil tersebut menunjukkan masih kurangnya peran serta masyarakat untuk mencegah DD dengan membasmi tempat perkembangbiakannya dan Kota Bandung masih berpotensi untuk terjadi penyebaran penyakit DD.

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Dengue hemorrhagic fever (DHF) is still a major public health problem in many regions in Indonesia including Bandung City. Community participation in implementing Eradication of Mosquito-Breeding Sites is still needed as it is considered the most effective method in controlling the dengue fever. This study aimed to determine the influence of health education towards community participation to eradicate mosquito-breeding sites, which was measured by knowledge level and larva index conducted in Bandung, specifically in two different locations with the highest and the lowest incidence rates of DHF. This study used quasi-experimental method. Samples were 100 people living in Cijawura and Cisaranten Wetan Subdistricts taken by using purposive sampling technique. Pre-test results showed that knowledge level of respondents in both subdistricts was significantly increasing (p value = 0.000) after health education was given. Container index (CI) and House index (HI) values in Cijawura Subdistrict were 13.2% and 26.7% respectively, then 9.6% and 28.4% respectively in Cisaranten Wetan Subdistrict. After the health education, CI value in Cijawura and Cisaranten Wetan Subdistricts significantly decreased (p value < 0.05), but HI value did not (p value > 0.05). Keywords: Dengue hemorrhagic fever, health education, larva index, knowledge Abstrak Penyakit demam berdarah dengue (DBD) masih menjadi masalah kesehatan utama di beberapa wilayah di Indonesia, termasuk Kota Bandung. Partisipasi masyarakat dalam melaksanakan Pemberantasan Sarang Nyamuk (PSN) sangat diperlukan karena PSN masih menjadi metode pengendalian DBD yang paling efektif. Penelitian ini bertujuan untuk mengetahui pengaruh pendidikan kesehatan terhadap partisipasi masyarakat dalam memberantas sarang nyamuk Aedes aegypti yang diukur dari tingkat pengetahuan dan indikator larva index yang dilakukan di Kota Bandung di dua lokasi berbeda dengan kasus DBD tertinggi dan terendah. Penelitian ini merupakan penelitian quasi experimental. Besar sampel sebanyak 100 orang yang tinggal di Kelurahan Cijawura dan Cisaranten Wetan diperoleh melalui teknik purposive sampling. Hasil pre-test menunjukkan bahwa tingkat pengetahuan responden di dua kelurahan tersebut meningkat secara signifikan (nilai p = 0,000) setelah pendidikan kesehatan diberikan. Nilai container index (CI) dan house index (HI) saat sebelum tes di Kelurahan Cijawura adalah 13.2% dan 26.7% dan Kelurahan Cisaranten Wetan adalah 9.6% dan 28.4%. Setelah dilakukan penyuluhan, nilai CI di Kelurahan Cijawura maupun Cisaranten Wetan dapat menurun secara signifikan (nilai p < 0,05) namun tidak untuk HI (nilai p > 0,05).

Background: Incidence rate of dengue hemorrhagic fever (DHF) in Bandung city is still considerably high over the years. There is no well-documented example of the effectiveness of fogging focus and there is no data on fogging focus activities that have been undertaken by Dinas Kesehatan Kota Bandung. Therefore, this study aims to analyze the relationship between frequency of fogging focus and incidence of DHF cases in Bandung from year 2010 to 2015.Methods: An analytical observational study with cross sectional design was conducted from September 2016 to November 2016. Population of this research was all DHF patients in Bandung. Data collected was analyzed using Spearman correlation coefficient.Results: Correlation between frequency of fogging focus and incidence of DHF cases was significant (p<0.05). Correlation coefficient of 0.703 is indicative of a strong positive correlation between the two variables.Conclusions: Frequency of fogging focus and incidence of DHF cases are positively correlated. This indicates that the higher the frequency of fogging focus, the higher the incidence of DHF cases.