A novel electrochemical sensor based on pyrrolidinium ionic liquid-ordered mesoporous carbon composite system for the determination of carbendazim was developed.
Viral
outbreaks have caused great disruptions to the economy and
public health in recent years. The accurate detection of viruses is
a key factor in controlling and overcoming epidemics. In this study,
an ultrasensitive molecularly imprinted virus sensor was developed
based on an “explosive” secondary amplification strategy.
Magnetic particles coated with carbon quantum dots (Fe3O4@CDs) were used as carriers and fluorescent probes,
while aptamers were introduced into the imprinting layer to enhance
the specific recognition of the target virus enterovirus 71 (EV71).
When EV71 was captured by the imprinted particles, the fluorescence
of the CDs was quenched, especially after binding to the aptamer-modified
ZIF-8 loaded with a large amount of phenolphthalein, thereby resulting
in signal amplification. Then, when adjusting the pH of the solution
to 12, the decomposition of ZIF-8 released phenolphthalein, which
turned the solution red, leading to the second “explosive”
amplification of the signal. Therefore, the detection of EV71 with
ultrasensitivity was achieved, which allows for visual detection by
the naked eye in the absence of any instruments. The detection limits
for fluorescence and visualization detection were 8.33 fM and 2.08
pM, respectively. In addition, a satisfactory imprinting factor of
5.4 was achieved, and the detection time only needed 20 min. It is
expected that this fluorescence-colorimetric dual-mode virus molecularly
imprinted sensor will show excellent prospects in epidemic prevention
and rapid clinical diagnosis.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.