ABSTRACT. A simple, sensitive and accurate spectrophotometric method for the determination of tranexamic acid (TXA) has been developed. The method was based on the charge transfer reaction between TXA as n-electron donor and 7,7,8,8-tetracyanoquinodimethane (TCNQ) asacceptor resulting in highly coloured stable complex, which showed maximum absorption band at wavelength of 419 nm. The formation of such complex was also confirmed by infrared measurement. The stoichiometry of the complex was found to be 1:1 between donor and acceptor as determined by Job's continuous variation method and straight line method. The thermodynamic parameters were calculated as association constant KCT of 5109 mol −1 and Gibbs free energy ∆G° of 22.22 kJ mol −1 . Different variables affecting the CT reaction were carefully studied and optimized. At the optimum reaction conditions, Beer's law was obeyed in a concentration range of 1−18 µg mL −1 of TXA using TCNQ reagent and the limit of detection was 0.2 µg mL −1 . Different analytical parameters, namely molar absorptivity (ε), relative standard deviation, correlation coefficient, limit of detection, and Sandell sensitivity, were calculated. The developed method could be applied successfully for the determination of TXA in its pharmaceutical dosage forms with a good precision and accuracy compared to the official method.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.