Background Potent immune-suppressive therapy has been demonstrated to increase the risk of infective endocarditis (IE) in renal recipients. Reports of Corynebacterium striatum ( C. striatum ) endocarditis in renal recipients are scarce, thus limiting understanding of the disease. Case Presentation We describe a case of native valve endocarditis caused by C. striatum in a 35-year-old male patient. The young man with end-stage renal failure underwent kidney transplantation because of autosomal dominant polycystic kidney disease. Ceftazidime was administered after the surgery according to routine procedures, and the patient was discharged on the 14th day after the surgery without any evidence of infection. The patient experienced fever on the 56th day, and Corynebacterium was cultured from the patient’s blood, in agreement with the results of testing of the donor kidney preservation solution. On the 64th day, multiple thromboses were found in the right external iliac artery, particularly around the anastomotic orifice of the transplanted renal artery. Vegetation was found in the posterior mitral valve tip on the 65th day. The patient had symptoms of persistent angina pectoris and chest tightness and underwent mitral valve replacement and vegetative resection. The patient eventually died. C. striatum was detected in the mitral valve and vegetation tissue with metagenomic next-generation sequencing. Conclusion C. striatum may cause endocarditis and endanger patients’ lives and thus warrants greater attention. Genotypic assays such as metagenomic next-generation sequencing are demonstrated to be effective in confirming species identity. Adequate anti-infection therapy and early surgery are required after IE is discovered.
Background Tumor microenvironment are involved in the progression of prostate cancer as an "accomplice", and cancer cell–secreted exosomes were identified as crucial messengers can carry lncRNAs to participate in intercellular communication. Herein, we report that an lncRNA HOXA-AS3, was correlated positively with the castration resistance and progression of prostate cancer. Methods Indirect co-culture of PC-3 and LNCaP was performed to explore the androgen resistance of prostate cancer. MTT, colony formation test, exosome isolation, identification and uptake test, and western blot confirmed that exosomes promote androgen resistance in LNCaP cells. Further, the molecular mechanism of HOXA-AS3 was proved by bioinformation analysis, Dual-Luciferase Reporter Gene Assays, gene knockout and overexpression experiment, cell proliferation and apoptosis experiment, qPCR, immunofluorescence experiment and rescue experiment. Finally, rats xenografts and prostate tissue section were used to examine the role of HOXA-AS3 in the transformation of ADPC into CRPC. Results Exosome-derived lncRNA HOXA-AS3 promotes the proliferation, migration, invasion and hormone resistance of prostate cancer cells. HOXA-AS3 functions as a competing endogenous RNAs (ceRNA) for miRNA-29b-3p, thereby elevating Mcl-1 and STAT3 expression, inhibiting the release of cytochrome c and the activation of Caspases-9, resulting in the decrease of hormone sensitivity, and finally leads to androgen resistance and progression of prostate cancer. Conclusions lncRNA HOXA-AS3 participates in the castration resistance and progression of prostate cancer through regulating the miR-29b-3p/Mcl-1/STAT3 pathway. lncRNA HOXA-AS3 might have the potential to serve as a candidate intervention targets for prostate cancer treatment.
Background: We aimed to introduce our modified hand-assisted retroperitoneoscopic living donor nephrectomy (HARPLDN) technique and define the learning curve. Methods: One hundred thirty-eight kidney donors who underwent modified HARPLDN by the same surgeon between May 2015 and March 2022 were included. A cumulative sum (CUSUM) learning curve analysis was performed with the total operation time as the study outcome. Results: In total, the mean operative time was 138.2±32.1 min. The median warm ischemic time (WIT) and estimated blood loss were 90 s and 50 ml, respectively. The learning curve for the total operative time was best modeled as a second-order polynomial with the following equation: CUSUMOT (min) = (–0.09 case number2) + (12.88 case number) – 67.77 (R2 = 0.7875; p<0.05). The CUSUM learning curve included the following three unique phases: phase 1 (the initial 41 cases), representing the initial learning curve; phase 2 (the middle 43 cases), representing expert competence; and phase 3 (the final 54 cases), representing mastery. The overall 6-month graft survival rate was 99.3%, with 94.9% immediate onset of graft function without delayed graft function and 0.7% ureteral complications. Conclusions: Our modified method is safe and effective for living donor nephrectomy and has the advantages of a shorter operating time and optimized WIT. The surgeon can become familiar with the modified HARPLDN after 41 cases and effectively perform the next 97 cases.
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