The inactivation kinetics of enzymes is an important way to reveal the mechanism of enzyme inactivation by microwaves. In this paper, lipase (LA) and lipoxygenase (LOX) were treated by both microwave irradiation and conventional heating. A temperature‐controlled water bath was used for conventional heating, and a microwave monomode reactor with a simultaneous cooling and precise temperature control system was used for microwave irradiation. The results showed that the effects of both microwave irradiation and conventional heating on enzyme inactivation were enhanced as system temperature increased; LOX was more sensitive to heat than LA. Therefore, compared with conventional heating, microwave irradiation shows no significant differences in inactivating LA or LOX in aqueous solution, and the thermal effect of microwave irradiation is the main cause of enzyme inactivation. Practical Applications Wheat germ has a short shelf life because of the high activity of endogenous enzymes. Two relevant endogenous enzymes are involved in the degradation of nutrients, lipase (LA) and lipoxygenase (LOX); therefore, the inactivation of LA and LOX is an essential prerequisite for long‐term storage of wheat germ. Microwave irradiation treatment (MIT) has long been applied to the inactivation of enzymes and has several advantages such as having a minimal effect on food product quality and providing rapid and uniform heating. However, the mechanism of enzyme inactivation by microwaves is still controversial. In this study, wheat germ endogenous enzymes were treated by MIT and conventional heat treatment. Based on residual enzyme activities and first‐order rate of inactivation kinetics, the mechanism of microwave inactivation was demonstrated, offering a theoretical basis for the future application of this technique in grain stabilization.
Principle of the pipeline leak detection system is presented, and the leak detection method based on acoustic wave and wavelet analysis is studied in this paper. The dynamic pressure transmitter based on piezoelectric dynamic pressure transducer is designed. The characteristic of dynamic pressure transmitter when pipeline leak happened is analyzed. The dynamic pressure signal is suitable for pipeline leak detection for quick-change of pipeline internal pressure, while the static pressure is suitable for slow-change of pipeline internal pressure. The signal is analyzed by wavelet analysis method to detect the singularity, and the singularity is used to recognize and locate the leak. This paper indicated that the dynamic pressure signal could be adjust to this detection that the pressure changes in the pipeline. Field tests in 68.2 km pipeline segment show that the method detects pipeline leak rapidly and precisely.
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