Docosahexaenoic acid (DHA) production in Schizochytrium sp. HX-308 was evaluated by detecting enzymatic activities of ATP:citrate lyase (EC 4.1.3.8), malic enzyme (EC 1.1.1.40) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) at different fermentation stages. According to the analysis, a regulation strategy was proposed which reinforced acetyl-CoA and NADPH supply at a specific fermentation stage. DHA content of total fatty acids was increased from 35 to 60% by the addition of 4 g/L malic acid at the rapid lipid accumulation stage. Total lipid content also showed an apparent increase of 35% and reached 19 g/L when 40 mL ethanol/L was added at the late lipid accumulation stage.

Temperature was one of the important environmental factors affecting the biosynthesis of docosahexaenoic acid (DHA; C22:6, ω-3). Generally, a low temperature would slow the strain growth, but promote the accumulation of unsaturated fatty acids. According to this information, the effects of temperature and different two-stage temperature shifting strategies on fatty acid production and DHA content of the marine fungoid protist, Schizochytrium sp. HX-308, were investigated in this study. Finally, the highest DHA percentage was up to 51.98% (per total fatty acids) with the DHA production of 6.05% (per dry cell weight), which was obtained with the method of shifting temperature from 30 °C for 32 h to 20 °C for 12 h.

The present study focused on improving docosahexaenoic acid (DHA) production by Schizochytrium sp. through N-methyl-N-nitro-N-nitrisiguanidine treatment coupled with ultraviolet radiation based on the metabolic pathway analysis. The activity of glucose-6-phosphate dehydrogenase of the mutant was higher than the parent strain, which indicated that the hexose monophosphate pathway of the mutant was strengthened, and more NADPH was thus produced. Also, the activities of malic enzyme and ATP-citrate lyase in the cell extract of the mutant were higher than the parent strain, which indicated that the screening method increased NADPH and acetyl-CoA supply in vivo effectively. Finally, in the batch culturing of the mutant, 34.84% higher lipid was accumulated with the cell dry weight at the same level compared with the parent strain. Moreover, the DHA percentage of the total fatty acids up to 56.22% was achieved using the mutant, which was 38.88% higher than the parent strain. When the cultures were maintained under appropriate conditions, the final DHA yield was 0.20 and 0.11 g/g dry biomass, for the mutant and parent, respectively.

With a perturbative treatment based on the Keldysh Green's function technique, we study the resonant enhancement of nonlinear interaction effects in a four-mode optomechanical ring. In such a system, we identify five distinct types of resonant scattering between unperturbed polariton modes, induced by the nonlinear optomechanical interaction. By computing the cavity density of states and optomechanical induced transparency signal, we find that the largest nonlinear effects are induced by a decay process involving the two phonon-like polaritons. In contrast to the conventional twomode optomechanical system, our proposed system can exhibit prominent nonlinear features even in the regime when the single-photon coupling is much smaller than the cavity damping.

Superconducting circuits with coupler architecture receive considerable attention due to their advantages in tunability and scalability. Although single-qubit gates with low error have been achieved, high-fidelity two-qubit gates in coupler architecture are still challenging. This paper pays special attention to examining the gate error sources and primarily concentrates on the related physical mechanism of ZZ parasitic couplings using a systematic effective Hamiltonian approach. Benefiting from the effective Hamiltonian, we provide simple and straightforward insight into the ZZ parasitic couplings that were investigated previously from numerical and experimental perspectives. The analytical results obtained provide exact quantitative conditions for eliminating ZZ parasitic couplings, and trigger four novel realizable parameter regions in which higher fidelity two-qubit gates are expected. Beyond the numerical simulation, we also successfully drive a simple analytical result of the two-qubit gate error from which the trade-off effect between qubit energy relaxation effects and ZZ parasitic couplings is understood, and the resulting two-qubit gate error can be estimated straightforwardly. Our study opens up new opportunities to implement high-fidelity two-qubit gates in superconducting coupler architecture. I. BACKGROUND AND MOTIVATION