Ischemic brain injury is acute local inflammation, leading to accumulation of pro-inflammatory cytokines. Cytokines influence the recruitment of leucocytes and play a key role in the inflammatory injury processes. Recently, a number of studies have demonstrated a close relationship between brain ischemia and cytokines. Interleukin-17 (IL-17) is a newly identified T-cell-specific cytokine. In this study, we evaluated the source and the action of IL-17 over the course of cerebral ischemia in rats (Sprague-Dawley) and humans. The levels of IL-17 in the ischemic hemisphere of the human brain, which was removed at necropsy, were assayed immunohistochemically. In rats, permanent middle cerebral artery occlusion (pMCAO) was obtained by inserting nylon monofilament into the right external carotid artery, occluding the right middle cerebral artery. The expression of IL-17 mRNA in rat was assayed using oligoprobe in situ hybridization. IL-17 production by neuroglial cells was assayed by double-staining using antibody glial fibrillary acidic protein (GFAP) and antibody IL-17. Levels of IL-17 were elevated in the ischemic hemispheres of human brain compared with the opposite normal hemispheres and peaked at days 3-5 after brain ischemia. The IL-17-positive cells were found in the ischemic lesion region. IL-17 mRNA was also elevated in ischemic hemispheres of pMCAO-operated rats, which were slightly elevated after 1 h and peaked at 6 days. IL-17 and GFAP double-stained were extensive in rat ischemic hemisphere. The ischemia-induced IL-17 expression in human brain reported here for the first time was very similar to that in rat model except that the peak was slightly earlier. We found for the first time that IL-17 was involved in an intense inflammatory reaction of brain ischemic injury in human. In pMCAO-operated rats, our findings suggest that IL-17 is produced by the neuroglial cells in the brain region undergoing ischemic insult. We suggest that in additional to T cells the neuroglial cell may be another cellular origin of IL-17 in later progression of brain ischemia.
A uniform nanolayer of europium-doped Gd2O3 was coated on the surface of preformed submicron silica spheres by a Pechini sol-gel process. The resulted SiO2 @ Gd2O3:Eu3+ core-shell structured phosphors were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), photoluminescence (PL) spectra as well as kinetic decays. The XRD results show that the Gd2O3:Eu3+ layers start to crystallize on the SiO2 spheres after annealing at 400 degrees C and the crystallinity increases with raising the annealing temperature. The core-shell phosphors possess perfect spherical shape with narrow size distribution (average size: 640 nm) and non-agglomeration. The thickness of the Gd2O3:Eu3+ shells on the SiO2 cores can be adjusted by changing the deposition cycles (70 nm for three deposition cycles). Under short UV excitation, the obtained SiO2@Gd2O3:Eu3+ particles show a strong red emission with 5D0-7F2 (610 nm) of Eu3+ as the most prominent group. The PL intensity of Eu3+ increases with increasing the annealing temperature and the number of coating cycles.
Zeolitic mineral admixture (ZMA) is made of the finely divided powder of natural zeolite with a bit of other agent such as triethanolamine. When ZMA is used to displace about 10% (by mass) of the ordinary portland cement (OPC) (strength grade No. 525) in concrete and mixed with a suitable amount of superplasticizer (W/C = 0.31 to 0.35), then a high-strength concrete with compressive strength of about 80 MPa and a slump of about 180 mm can be obtained. The strength of this concrete is about 10 to 15% higher than that of the corresponding concrete mixed with pure OPC, and its bleeding decreases greatly. It also results in no segregation or separation of the mix, and thus it satisfies the requirement of pumping concrete in construction. The ZMA is suitable not only for the OPC but also for the slag portland cement. The strengthening effect of the ZMA is somewhat similar to that of silica fume. But the cost is only two thirds that of OPC. Thus, when ZMA is used to displace a certain amount of the cement in the concrete, the cost of the concrete thus made will be 3 to 5% cheaper than that of the corresponding concrete with pure cement. The ZMA can increase the amount of micropores (d < 625 Å) and decrease the amount of harmful large pores (d > 938 Å) in the cement paste. Hence, the strength of concrete is increased and its other properties are also improved. Furthermore, ZMA can raise the SiO2/CaO weight ratio in the transition zone to increase its C-S-H phase and decrease its calcium hydroxide content. Thus, the structure of the transition zone is improved. Consequently, the strength and resistance to permeability of the concrete are increased.
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