Abstract— –Phycoerythrobilin dimethyl ester (PEBDM) or isomesobiliviolin dimethyl ester (IBVDM) formed complexes with zinc ions in chloroform. The complex ratios were determined to be 1:1 according to the spectrophotometric titration of PEBDM and IBVDM with zinc ions in methanol and the mass spectra of the complexes. The chromophore compositions of R‐phycoerythrin from Porphyra yezoensis were determined by spectrophotometric titration of α‐ and β‐subunits at pH 13.0 with zinc ions. There were 2 phycoerythrobilins in the a‐subunit, and 1 phycourobilin and 3 phycoerythrobilins in the β‐subunit.
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