High-resolution mass spectrometry is currently used to determine the mass of biologically active compounds in medicinal plants and food and UHPLC-Orbitrap is a relatively new technology that allows fast fingerprinting and metabolomics analysis. Forty-two metabolites including several phenolic acids, flavonoids, coumarines, tremetones and ent-clerodane diterpenes were accurately identified for the first time in the resin of the medicinal plant (Asteraceae) a Chilean native species, commonly called umatola, collected in the pre-cordillera and altiplano regions of northern Chile, by means of UHPLC-PDA-HR-MS. This could be possible by the state of the art technology employed, which allowed well resolved total ion current peaks and the proposal of some biosynthetic relationships between the compounds detected. Some mayor compounds were also isolated using HSCCC. The ethanolic extract showed high total polyphenols content and significant antioxidant capacity. Furthermore, several biological assays were performed that determined the high antioxidant capacity found for the mayor compound isolated from the plant, 11--coumaroyloxyltremetone.
CCDC no.: 1504400The title crystal structure (sytematic name: 2-(5-acetyl-2,3-dihydrobenzofuran-2-yl)allyl (E)-3-(4-hydroxyphenyl) acrylate) is shown in the gure. Tables 1 and 2 contain details of the measurement method and a list of the atoms including atomic coordinates and displacement parameters. gummy extract was obtained. A portion of the extract (16 g) was ltered and submitted to a medium pressure column chromatography system composed of an 2.5 cm x 48 cm medium pressure column (Aceglass inc, Vineland, NY, USA) packed with silicagel (Kieselgel 60 H, Merck, Darmstadt, Germany) using an isocratic solvent system of hexane-ethyl acetate (8:2 v:v) pumped with a medium pressure pump (FMI lab pump, Syosset, NY, USA) with a ow rate of 10 mLminute. The fractions were collected using a Gilson FC 203B fraction collector (Gilson, Middleton, MI, USA) and the volume of each fraction was 5 mL·The collected fractions (140) were combined according to TLC analysis (Kieselgel F254 plates, developed with CHCl 3 : EtOAc 18:2 v/v, and spots visualized by spraying with vanillin: sulfuric acid 2% in ethanol and heating) and 17 combined fractions were obtained. Fraction eight (1.5 g) was re-chromatographed using the same system and 22 mg of umbelliferone [1] were isolated. Fraction 11 (3.5 g) was chromatographed using the mentioned system and the title compound [2] was isolated (525 mg). Suitable crystals for a crystallographic study were obtained after re-crystallization from hexane: ethyl acetate 9:1 v/v. The structure was already reported, however, we have revised and corrected NMR data [1] with the Source of material
CCDC no.: 1526063The asymmetric unit of the title crystal structure is shown in the gure. Tables 1 and 2 contain details on crystal structure and measurement conditions and a list of the atoms including atomic coordinates and displacement parameters.
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