Diesel fuel is one of the most important sources of hydrocarbon contamination worldwide. Its composition consists of a complex mixture of n-alkanes, branched alkanes and aromatic compounds. Hydrocarbon degradation in Pseudomonas species has been mostly studied under aerobic conditions; however, a dynamic spectrum of oxygen availability can be found in the environment. Pseudomonas extremaustralis, an Antarctic bacterium isolated from a pristine environment, is able to degrade diesel fuel and presents a wide microaerophilic metabolism. In this work RNA-deep sequence experiments were analyzed comparing the expression profile in aerobic and microaerophilic cultures. Interestingly, genes involved in alkane degradation, including alkB, were over-expressed in micro-aerobiosis in absence of hydrocarbon compounds. In minimal media supplemented with diesel fuel, n-alkanes degradation (C13-C19) after 7 days was observed under low oxygen conditions but not in aerobiosis. In-silico analysis of the alkB promoter zone showed a putative binding sequence for the anaerobic global regulator, Anr. Our results indicate that some diesel fuel components can be utilized as sole carbon source under microaerophilic conditions for cell maintenance or slow growth in a Pseudomonas species and this metabolism could represent an adaptive advantage in polluted environments.
In Buenos Aires periurban area, horticultural practices are one of the most important activities. Pesticides and fertilisers are used without any control to cover the farmers' needs, obtaining high crop yields at short terms and modifying soil ecosystem in the long term. The aim of this work was to isolate indigenous strains from periurban horticultural units with pesticide degrading capacity and to evaluate their plant growth-promoting properties in order to design biofertilisers to be applied in the restoration of these exploited soils. After the screening, eight strains were isolated and identified. They showed not only the capacity to produce indole-3-acetic acid, to fix nitrogen, to secrete siderophores and to solubilise calcium phosphate but also tolerated the mixture of pesticides usually used for horticultural practices. By their behaviour in mixed cultures and plant growth-promoting properties, these autochthonous isolates represent a promising alternative as biofertilisers according to soil type and activity.
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