Ancestral obesogenic exposure is able to trigger harmful effects in the offspring left ventricle (LV) which could lead to cardiovascular diseases. However, the impact of the father’s lifestyle on the offspring LV is largely unexplored. The aim of this study was to investigate the effects of 8 weeks of paternal resistance training (RT) on the offspring left ventricle (LV) proteome exposed to control or high-fat (HF) diet. Wistar rats were randomly divided into two groups: sedentary fathers and trained fathers (8 weeks, 3 times per week with weights secured to the animals’ tails). The offspring were obtained by mating with sedentary females. Upon weaning, male offspring were divided into 4 groups (5 animals per group): offspring from sedentary fathers, exposed to control diet (SFO-C); offspring from trained fathers, exposed to control diet (TFO-C); offspring from sedentary fathers, exposed to high-fat diet (SFO-HF); and offspring from trained fathers, exposed to high-fat diet (TFO-HF). The LC-MS/MS analysis revealed 537 regulated proteins among groups. Offspring exposure to HF diet caused reduction in the abundance levels of proteins related to cell component organization, metabolic processes, and transport. Proteins related to antioxidant activity, transport, and transcription regulation were increased in TFO-C and TFO-HF as compared with the SFO-C and SFO-HF groups. Paternal RT demonstrated to be an important intervention capable of inducing significant effects on the LV proteome regardless of offspring diet due to the increase of proteins involved into LV homeostasis maintenance. This study contributes to a better understanding of the molecular aspects involved in transgenerational inheritance.

The increase in high-energy dietary intakes is a well-known risk factor for many diseases, and can also negatively impact the tendon. Ancestral lifestyle can mitigate the metabolic harmful effects of offspring exposed to high-fat diet (HF). However, the influence of paternal exercise on molecular pathways associated to offspring tendon remodeling remains to be determined. We investigated the effects of 8 weeks of paternal resistance training (RT) on offspring tendon proteome exposed to standard diet or HF diet. Wistar rats were randomly divided into two groups: sedentary fathers and trained fathers (8 weeks, three times per week, with 8-12 dynamic movements per climb in a stair climbing apparatus). The offspring were obtained by mating with sedentary females. Upon weaning, male offspring were divided into four groups (five animals per group): offspring from sedentary fathers were exposed either to control diet (SFO-C), or to high-fat diet (SFO-HF); offspring from trained fathers were exposed to control diet (TFO-C) or to a high-fat diet (TFO-HF). The Nano-LC-MS/MS analysis revealed 383 regulated proteins among offspring groups. HF diet induced a decrease of abundance in tendon proteins related to extracellular matrix organization, transport, immune response and translation. On the other hand, the changes in the offspring tendon proteome in response to paternal RT were more pronounced when the offspring were exposed to HF diet, resulting in positive regulation of proteins essential for the maintenance of tendon integrity. Most of the modulated proteins are associated to biological pathways related to tendon protection and damage recovery, such as extracellular matrix organization and

RESUMO O objetivo deste estudo foi identificar, a partir de uma revisão sistemática, variáveis clínicas, instrumentais e demográficas associadas com maior risco de queda em indivíduos com esclerose múltipla (EM), com base em dados prospectivos. A pesquisa foi conduzida nas bases de dados Medline, Web of Science, Bireme e CINAHL, utilizando os descritores “esclerose múltipla”, “quedas”, “quedas acidentais”, “risco de quedas”, “controle postural” e “equilíbrio”, seguido de buscas manuais. Foram considerados elegíveis estudos de coorte prospectivos, com período mínimo de acompanhamento das quedas de três meses, que avaliassem a associação de determinada variável demográfica, clínica ou instrumental em relação a maior risco de queda em indivíduos com EM. A escala de Newcastle-Ottawa modificada foi utilizada para avaliação da qualidade metodológica dos estudos incluídos. Foram identificados 357 estudos, 12 dos quais foram incluídos na revisão sistemática, com total de 1.270 pacientes incluídos. Destes, 740 (58,26%) pacientes apresentaram um ou mais episódios de queda, 396 (31,18%) apresentaram episódios de queda recorrentes (2≥quedas no período estipulado), e 530 (41,74%) não apresentaram nenhum episódio. Com exceção da espasticidade e do impacto da dupla tarefa na velocidade da marcha, todas as variáveis investigadas apresentaram resultados conflitantes quanto às suas associações a maior risco de quedas. São necessários mais estudos que apresentem homogeneidade dos fenótipos clínicos de indivíduos com EM, além da utilização de instrumentos de avaliação validados, a fim de estabelecer uma associação robusta de outras variáveis clínicas, instrumentais e demográficas com maior risco de queda.

This study analyzed the timed up and go test (TUG) as a tool to evaluate muscle strength in community-dwelling older people. As a methodology, an observational, cross-sectional and analytical study was conducted. Data from 442 older people were analyzed, both women (71.7%) and men (28.3%). Ages ranged from 62- 104 years, with a mean of 80.85 (±8.047) years. TUG was used as a tool to evaluate muscle strength. Comparison and correlation analyses were performed to compare performance between the TUG and the chair stand test (CST) and between TUG and handgrip strength (HGS). Analysis of agreement between the diagnosis of low physical performance on TUG and the diagnosis of muscle weakness on CST and HGS was also performed. The predictive values were estimated using the receiver operating characteristic curve. The analyses showed a moderate direct proportional correlation between TUG and CST performance and low inversely proportional correlations between TUG and HGS performance and between CST and HGS (p<0.001). In the accuracy analyses, it was observed that TUG test could predict muscle strength in CST (S 34.4%; PPV 91.5%; NPV 26.1%; AUC=0.779; 95%CI 0.724-0.835; p<0.001) and handgrip dynamometry (S 44.2%; PPV 77.4%; NPV 67.2%; AUC=0.652; 95%CI 0.599-0.705; p<0.001). The Timed Up and Go test was able to satisfactorily indicate individuals with normal muscle strength in CST.

RESUMO Este estudo analisou o uso do timed up and go test (TUG) como ferramenta de avaliação da força muscular em idosos da comunidade. Como metodologia, foi realizado um estudo observacional, transversal e analítico. Foram analisados dados de 442 idosos, do sexo feminino (71,7%) e do masculino (28,3%), com idades entre 62 e 104 anos, e média de 80,85 (±8,047) anos. O TUG foi utilizado como instrumento de avaliação da força muscular. Foram realizadas análises de comparação e correlação entre o desempenho no TUG e no teste de sentar e levantar (TSL), e entre o TUG e a força de preensão manual. Também foram realizadas análises de concordância entre diagnóstico de baixo desempenho físico no TUG e diagnóstico de fraqueza muscular no TSL e na força de preensão manual. Os valores preditivos foram traçados por meio da curva receiver operating characteristic (ROC). As análises mostraram correlação diretamente proporcional moderada entre o desempenho no TUG e no TSL e correlações inversamente proporcionais baixas entre o desempenho no TUG e a força de preensão manual e entre o TSL e a força de preensão manual (p<0,001). Nas análises de acurácia, observou-se que o TUG apresentou capacidade de prever a força muscular no TSL (S 34,4%; VPP 91,5%; VPN 26,1%; AUC=0,779; 95% IC 0,724-0,835; p<0,001) e na dinamometria de preensão palmar (S 44,2%; VPP 77,4%; VPN 67,2%; AUC=0,652; 95% IC 0,599-0,705; p<0,001). Conclui-se que o TUG apresentou capacidade de indicar de forma satisfatória os indivíduos com força muscular normal no TSL.