An extracellular protease of a mutant of strain 8325N of Staphylococcus aureus, was isolated by a three-step procedure involving stepwise chromatography on DEAE-cellulose, gradient chromatography on hydroxyapatite and gel filtration on Sephadex G-150. The enzyme was purified 250 times to homogeneity with a recovery of 20-30°/,.The protease has a molecular weight of about 29000 and consists of a single polypeptide chain as shown by gel filtration of native enzyme on calibrated columns of Sephadex G-150 as well as gel filtration of denaturated enzyme on Sepharose 6-B in 6 M guanidine-HC1 and dodecylsulfatepolyacrylamide gel electrophoresis. Peptide maps also suggest a molecular weight around 30000.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.