Cyclic dinucleotides are second messengers in the cyclic
GMP–AMP
synthase (cGAS)–stimulator of interferon genes (STING) pathway,
which plays an important role in recognizing tumor cells and viral
or bacterial infections. They bind to the STING adaptor protein and
trigger expression of cytokines via TANK binding kinase 1 (TBK1)/interferon
regulatory factor 3 (IRF3) and inhibitor of nuclear factor-κB
(IκB) kinase (IKK)/nuclear factor-κB (NFκB) signaling
cascades. In this work, we describe an enzymatic preparation of 2′–5′,3′–5′-cyclic
dinucleotides (2′3′CDNs) with use of cyclic GMP–AMP
synthases (cGAS) from human, mouse, and chicken. We profile substrate
specificity of these enzymes by employing a small library of nucleotide-5′-triphosphate
(NTP) analogues and use them to prepare 33 2′3′CDNs.
We also determine affinity of these CDNs to five different STING haplotypes
in cell-based and biochemical assays and describe properties needed
for their optimal activity toward all STING haplotypes. Next, we study
their effect on cytokine and chemokine induction by human peripheral
blood mononuclear cells (PBMCs) and evaluate their cytotoxic effect
on monocytes. Additionally, we report X-ray crystal structures of
two new CDNs bound to STING protein and discuss structure–activity
relationship by using quantum and molecular mechanical (QM/MM) computational
modeling.
Cyclic dinucleotides (CDNs) are second messengers that activate stimulator of interferon genes (STING). The cGAS-STING pathway plays a promising role in cancer immunotherapy. Here, we describe the synthesis of CDNs containing 7-substituted 7-deazapurine moiety. We used mouse cyclic GMP−AMP synthase and bacterial dinucleotide synthases for the enzymatic synthesis of CDNs. Alternatively, 7-(het)aryl 7-deazapurine CDNs were prepared by Suzuki−Miyaura cross-couplings. New CDNs were tested in biochemical and cell-based assays for their affinity to human STING. Eight CDNs showed better activity than 2′3′-cGAMP, the natural ligand of STING. The effect on cytokine and chemokine induction was also evaluated. The best activities were observed for CDNs bearing large aromatic substituents that point above the CDN molecule. We solved four X-ray structures of complexes of new CDNs with human STING. We observed π−π stacking interactions between the aromatic substituents and Tyr240 that are involved in the stabilization of CDN-STING complexes.
Chronic
hepatitis B (CHB) remains a major public health problem
worldwide, with limited treatment options, but inducing an antiviral
response by innate immunity activation may provide a therapeutic alternative.
We assessed the cytokine-mediated anti-hepatitis B virus (HBV) potential
for stimulating the cyclic GMP–AMP synthase–stimulator
of interferon genes (STING) pathway using STING agonists in primary
human hepatocytes (PHH) and nonparenchymal liver cells (NPCs). The
natural STING agonist, 2′,3′-cyclic GMP–AMP,
the synthetic analogue 3′,3′-c-di(2′F,2′dAMP),
and its bis(pivaloyloxymethyl) prodrug had strong indirect cytokine-mediated
anti-HBV effects in PHH regardless of HBV genotype. Furthermore, STING
agonists induced anti-HBV cytokine secretion in vitro, in both human and mouse NPCs, and triggered hepatic T cell activation.
Cytokine secretion and lymphocyte activation were equally stimulated
in NPCs isolated from control and HBV-persistent mice. Therefore,
STING agonists modulate immune activation regardless of HBV persistence,
paving the way toward a CHB therapy.
The liver is a complex organ that governs many types of metabolisms, including energy metabolism and other cellular processes. The liver also plays a crucial role in important functions in immunity, and the activity of liver tissue-associated immunity affects the outcome of many liver pathologies. A thorough characterization of the liver immune microenvironment may contribute to a better understanding of immune signaling, the mechanisms of specific immune responses, and even to improved predictions about therapy outcomes. In this paper, we present an optimized, simple, and rapid protocol to characterize the liver-associated immune cell milieu. We believe that the most suitable technique for obtaining a complex immune cell suspension and for removing contaminating blood cells is to perform mouse liver perfusion, using only phosphate buffer saline. Combining an enzymatic digestion and a mechanical dissociation of liver tissue, followed by cell purification, improves downstream applications. This combination is an essential prerequisite for immune cell determination and characterization. We then demonstrate a flow cytometry-based multiparametric immunophenotyping along with a gating strategy to detect and quantify liver endothelial cells, T cells (helper and cytotoxic), B cells, NK cells, NKT cells, neutrophils, monocytes (subsets included), dendritic cells (subsets included), macrophages and Kupffer cells.
Chronic hepatitis B (CHB) is caused by the Hepatitis B virus (HBV) and affects millions of people worldwide. Developing an effective CHB therapy requires using in vivo screening methods, such as mouse models reflecting CHB based on hydrodynamic delivery of plasmid vectors containing a replication-competent HBV genome. However, long-term expression of HBV proteins is accompanied by production of progeny virions, thereby requiring a Biosafety Level (BSL) 3 animal facility. In the present study, we introduced a point mutation in the START codon of the HBV polymerase to develop a mouse model reflecting chronic hepatitis B infection without formation of viral progeny. We induced the mouse model by hydrodynamic injection of adeno-associated virus plasmid vector (pAAV) and minicircle plasmid (pMC) constructs into C57Bl/6 and C3H/HeN mouse strains, monitoring HBV antigens and antibodies in blood by enzyme-linked immunosorbent assay and analyzing liver expression of HBV core antigen by immunohistology. Persisting expression of viral antigens over 140 days (study endpoint) was observed only in the C3H/HeN mouse strain when using pAAV/1.2HBV-A and pMC/1.0HBV-D with pre-C and pre-S recombination sites. In addition, pAAV/1.2HBV-A in C3H/HeN sustained HBV core antigen positivity up to the study endpoint in C3H/HeN mice. Moreover, introducing the point mutation in the START codon of polymerase effectively prevented the formation of viral progeny. Our study establishes an accessible and affordable experimental paradigm for developing a robust mouse model reflecting CHB suitable for preclinical testing of anti-HBV therapeutics in a BSL2 animal facility.
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