Highlights Inter-laboratory study with 174 participants using STRmix™ CE analysis settings resulted in larger differences in LR than PG software Differences in log(LR) due to MCMC variation were less than one order of magnitude Abstract (max 400 words)An intra and inter-laboratory study using the probabilistic genotyping (PG) software STRmix™ is reported. Two complex mixtures from the PROVEDIt set, analysed on an Applied Biosystems™ 3500 Series Genetic Analyzer, were selected. 174 participants responded.LRs were assigned, the point estimates ranging from 2 × 10 4 to 8 × 10 6 . For Sample 2 (in the order of 2000 rfu for major contributors), LRs ranged from 2 × 10 28 to 2 × 10 29 . Where LRs were calculated, the differences between participants can be attributed to (from largest to smallest impact): varying number of contributors (NoC), the exclusion of some loci within the interpretation, differences in local CE data analysis methods leading to variation in the peaks present and their heights in the input files used, and run-to-run variation due to the random sampling inherent to all MCMC-based methods.This study demonstrates a high level of repeatability and reproducibility among the participants. For those results that differed from the mode, the differences in LR were almost always minor or conservative.
Riding simulators are designed to replicate movement of a horse for the purpose of assessment and training of horse riders, but little is known about their similarity in replicating movement of horses. This study tested the validity of a dressage simulator, by measuring cycle/stride duration, range and symmetry of displacement of the simulator and comparing displacement vectors to that of real horses trotting on a treadmill. A reflective marker was placed on the midline of the simulator at the estimated level of the 18th thoracic vertebrae (T18), and over the T18 spinous process of ten horses. The simulator displacement was recorded in trot mode, while the real horses trotted at a comfortable speed on a treadmill. Displacements in three axes of motion were measured using 10 high-speed video cameras sampling at 240 Hz. Correlation tests showed high levels of statistical repeatability and symmetry of the simulator between multiple runs. Mean cycle/stride duration of the simulator was significantly faster than the group of horses by 0.17 s. Significant differences between the simulator and horses were shown in overall displacement in two axes, the simulator displaying 70% greater displacement in the mediolateral axis, 22% greater displacement in the craniocaudal axis, but displaying 12% less movement in the dorsoventral axis, which was not statistically significant. Displacement trajectories showed similarities in the frontal plane, displaying a butterfly-shaped sequence, but clear differences in the sagittal plane, with the horses showing an oval pattern of displacement and the simulator a clear linear displacement. Caution must therefore be taken with assumptions that riders will move in the same way on a simulator as they would on a real horse.
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