No abstract

No abstract

The aim of this study was to explore the synergistic effect of signal transducer and activator of transcription 3 (STaT3)-targeted small interfering (si)rna and aZd0530 against glioblastoma in vitro and in vivo. Glioblastoma cell lines u87 and u251 were divided into four groups and treated with control, lV-STaT3 sirna, aZd0530, and combined lV-STaT3 sirna with aZd0530, respectively. The proliferation and apoptotic capacity of glioblastoma cells was assessed by Cell Counting Kit-8 and double staining flow cytometry assays, respectively. additionally, the potential effect of lV-STaT3 sirna and aZd0530 on glioblastoma was evaluated in vivo. images were captured of the tumor formation in mice every week. Following three weeks of treatment, nMr scan and immunohistochemistry were performed. The treatment of combined lV-STaT3 sirna and aZd0530 was more effective in inhibiting proliferation and inducing apoptosisof glioblastoma cells in comparison with the treatment of either lV-STaT3 sirna or aZd0530 alone. although lV-STaT3 sirna or aZd0530 treatment alone suppressed tumor growth in mice, the combined treatment had a more significant effect than the treatment of lV-STaT3 sirna or aZd0530 alone. according to the results of both in vitro and in vivo assays, a combined therapy of lV-STaT3 sirna with aZd0530 could enhance therapeutic effects on glioblastoma, supporting the idea that the combination of lV-STaT3 sirna and aZd0530 could serve as a novel and effective strategy to combat glioblastoma.

ObjectiveClinical data on 61 patients (grouped by their treatment with MVD or RHZ) with glossopharyngeal neuralgia were analyzed retrospectively. A summary analysis of the effective rate and surgical complications of MVD and RHZ in the treatment of glossopharyngeal neuralgia was performed to observe the new surgical options for GN.MethodFrom March 2013 to March 2020, 63 patients with GN were admitted to our hospital by the professional group of cranial nerve diseases. Two patients diagnosed with tongue and pharynx pain secondary to tongue cancer and upper esophageal cancer, respectively were excluded from the group. The remaining patients all met the diagnosis of GN, some of them were treated with MVD and others were treated with RHZ. The pain relief rate, long-term results, and complications of the patients in the two groups were well-organized and analyzed.ResultOf the 61 patients, 39 were treated with MVD and 22 were treated with RHZ. In the early-stage patients (the first 23 patients), all of them were operated on with the MVD procedure except one patient without vascular compression. In the later-stage patients, MVD was performed for evident single arterial compression according to the intraoperative situation. And for compression of arteries with greater tension or PICA + VA complex compression, RHZ was performed. It was also performed in cases where vessels with tight adhesions to the arachnoid and nerves could not be easily separated, or where it was easy to damage the perforating arteries after separating the blood vessels, causing vasospasm, which affects the blood supply to the brainstem and cerebellum. RHZ was also performed if there was no clear vascular compression. The efficiency of both groups was 100%. In the MVD group, one case recurred 4 years after the initial operation, and RHZ was performed for reoperation. Complications related to the operation included one case of swallowing and coughing in the MVD group, and three cases in the RHZ group; two cases of uvula not centering in the MVD group, and five cases in the RHZ group. There was 2 patients in RHZ group lost taste in 2/3 of the backing of the tongue, though these symptoms mostly disappeared or decreased after follow-up. One patient in the RHZ group had developed tachycardia by the time of the long-term follow-up, but whether it was related to the surgery is still uncertain. In terms of serious complications, there were two cases of postoperative bleeding in the MVD group. Based on the clinical characteristics of the patients' bleeding, it was judged that the cause of the bleeding was ischemia and was related to an intraoperative injury to the penetrating artery of the PICA artery and vasospasm.ConclusionMVD and RHZ are effective methods for the treatment of primary glossopharyngeal neuralgia. MVD is recommended for cases where vascular compression is clear and easy to handle. However, for cases with complex vascular compression, tight vascular adhesions, difficult separation, and without clear vascular compression, RHZ could be performed. Its efficiency is equivalent to MVD, and there is no significant increase in complications such as cranial nerve disorders. There are few cranial nerve complications that seriously affect the quality of life of patients. RHZ helps to reduce the risk of ischemia and bleeding during surgery by reducing the risk of arterial spasms and injury to the penetrating arteries by separating the vessels due to separation of vessels during MVD. At the same time, it may reduce the postoperative recurrence rate.

The West Coast New Area of Qingdao has witnessed a fast-developing economy and accelerated urbanization since its establishment as a national new area, which also leads to the rapid change of land utilization status. In such a context, ways of quickly monitoring land utilization changes and leveraging land resources more efficiently and intensively are practical problem that requires urgent settlement. Focusing on Landsat8 OLI images in 2013, 2017 and 2021 of the West Coast New Area of Qingdao, data of land utilization changes in three periods were first extracted using ENVI5.3 software. And then, land utilization was dynamically monitored with the post classification comparison method. Finally, the driving force was quantitatively analyzed from the perspectives of socio-economic development, population change, and policy in combination with document literature collected, which can provide data supports for land planning of the West Coast New Area of Qingdao with suggestions proposed.