This study reports the GPU parallelization of complex three-dimensional software for nonlinear analysis of concrete structures. It focuses on coupled thermomechanical analysis of complex structures. A coupled FEM/DEM approach (CDEM) is given from a fundamental theoretical viewpoint. As the modeling of a large structure by means of FEM/DEM may lead to prohibitive computation times, a parallelization strategy is required. With the substantial development of computer science, a GPU-based parallel procedure is implemented. A comparative study between the GPU and CPU computation results is presented, and the runtimes and speedups are analyzed. The results show that dramatic performance improvements are gained from GPU parallelization.

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Despite the widely explored biomaterial scaffolds in vascular tissue engineering applications lately, no ideal platform has been provided for small diameter synthetic vascular grafts mainly due to the thrombosis issue. Endothelium is the only known completely non-thrombogenic material; so, functional endothelialization onto vascular biomaterials is critical in maintaining the patency of vascular networks. Bacterial cellulose (BC) is a natural biomaterial with superior biocompatibility and appropriate hydrophilicity as potential vascular grafts. In previous studies, surface modification of active peptides such as Arg-Gly-Asp (RGD) sequences onto biomaterials has been proven to achieve accelerated and selective endothelial cell (EC) adhesion. In our study, we demonstrated a new strategy to remotely regulate the adhesion of endothelial cells based on an oscillating magnetic field and achieve successful endothelialization on the modified BC membranes. In details, we synthesized bacterial cellulose (BC), magnetic BC (MBC), and RGD peptide-grafted magnetic BC (RMBC), modified with the HOOC-PEG-COOH-coated iron oxide nanoparticles (PEG-IONs). The endothelial cells were cultured on the three materials under different frequencies of an oscillating magnetic field, including "stationary" (0 Hz), "slow" (0.1 Hz), and "fast" (2 Hz) groups. Compared to BC and MBC membranes, the cells on RMBC membranes generally show better adhesion and proliferation. Meanwhile, the "slow" frequency of a magnetic field promotes this phenomenon on RMBC and achieves endothelialization after culture for 4 days, whereas "fast" inhibits the cellular attachment. Overall, we demonstrate a non-invasive and convenient method to regulate the endothelialization process, with promising applications in vascular tissue engineering.

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Real-time monitoring of newly acidified organelles during autophagy in living cells is highly desirable for a better understanding of intracellular degradative processes. Herein, we describe a reaction-based boron dipyrromethene (BODIPY) dye containing strongly electron-withdrawing diethyl 2-cyanoacrylate groups at the α-positions. The probe exhibits intense red fluorescence in acidic organelles or the acidified cytosol while exhibiting negligible fluorescence in other regions of the cell. The underlying mechanism is a nucleophilic reaction at the central meso-carbon of the indacene core, resulting in the loss of π-conjugation entailed by dramatic spectroscopic changes of more than 200 nm between its colorless, non-fluorescent leuco-BODIPY form and its red and brightly emitting form. The reversible transformation between red fluorescent BODIPY and leuco-BODIPY along with negligible cytotoxicity qualifies such dyes for rapid and direct intracellular lysosome imaging and cytosolic acidosis detection simultaneously without any washing step, enabling the real-time monitoring of newly acidified organelles during autophagy.

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Air pollution has caused serious public health concerns and attracted much attention of researchers, especially those working on sustainable and environmentally friendly polymers. In this work, we modified bacterial cellulose (BC) into N-acetyl BC (AcNBC) in an in situ manner and put it to use as filters for the removal of particulate matter (PM) particles. The structure and the morphology of BC-based materials were investigated by C NMR, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Through the observation of its microscopic morphology, we find that the nanoscale fiber diameter and the three-dimensional structure are almost impervious to the introduction of acetyl. The result of quantified PM removal by an AcNBC filter shows a higher removal of PM than that by pristine BC and meets the qualification of the 95% high-efficiency standard under high-concentration PM particles. Moreover, the BC-based filters after filtration showed favorable biodegradation in a real soil environment. These results suggest that BC-based materials can be effective candidate filters for the removal of polluted air, resulting in a healthier living environment.