Objective To examine the eCect of diCerent concensolution, resulted in a diminished response to all stimuli. This contractile dysfunction was least in the trations of calcium on the contractile responses of isolated strips of rabbit bladder detrusor to various presence of calcium chelator (EGTA) and greatest in the presence of 5.4 mmol/L calcium. Repetitive stimuforms of stimulation after 2 h incubation in the presence of substrate and oxygen depletion (in vitro ischaelation during in vitro ischaemia also exacerbated the contractile dysfunction. Lipid peroxidation increased mia), followed by 1 h of recovery. The resultant contractile responses were correlated with the level of during in vitro ischaemia in proportion to the calcium concentration and was enhanced by repetitive stimulalipid peroxidation as determined by malonedialdehyde (MDA) concentration.tion during this period. Regardless of the incubation conditions, the reduction in the contractile response Materials and methods Isolated strips of rabbit bladder detrusor smooth muscle were incubated in Tyrode's was significantly greater for field-stimulated tissues than for those stimulated with carbachol or KCl. solution containing diCerent concentrations of calcium (0-5.4 mmol/L). The eCect of 2 h of incubation inConclusions These results show that the magnitude of contractile dysfunction induced by incubation in the oxygen-and substrate-free medium (in vitro ischaemia), followed by a 1-h incubation in the presence of presence of substrate and oxygen depletion is reduced in the presence of low calcium concentrations, oxygen and substrate, on the contractile responses to field stimulation, carbachol and KCl were determined.increased in the presence of high calcium levels and increased in the presence of repetitive stimulation. In The eCects of repetitive stimulation (15 s of stimulation at 32 Hz applied every 5 min during the 2-h experaddition, the level of lipid peroxidation after the recovery period was proportional to the magnitude of imental period) were also assessed. Results The contractile responses to all stimuli increased contractile dysfunction present.
No abstract
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