Background: Ceratotheca triloba was found to contain three anthraquinones (9,3,10,) in its roots. Inhibition of the human topoisomerase II enzyme is the basis of some currently used cancer drugs such as doxorubicin which is shown to be cardio-toxic. For this reason we decided to investigate anthraquinones from C. triloba as a possible anticancer drug, however the main limitation was the large quantities of roots that are required to obtain a good yield of the active compound. Therefore the aim of this research was to obtain a higher yield of anthraquinones in hairy roots cultures than the parent plant as well as to compare yields of hairy root, cell suspension and shoot cultures. Materials and Methods: Protocols for seed sterilization, seed germination, shoot cultivation, callus induction, A. rhizogenes mediatedtransformation and hormone supplementations of hairy roots were developed. Results:The results revealed that stem explants was susceptible to transformation by Agrobacterium rhizogenes at a low optical density of 0.2. Induced hairy roots were decontaminated by exposure to cefotaxime at 500mg.l -1 for five days and then 200mg.l -1 for eight days. Visualization of culture extract profiles by TLC revealed anthraquinones were present in all cultures. Analysis of the culture extracts by HPLC showed the highest yield of anthraquinones was produced in hairy root cultures supplemented with 1-Naphthaleneacetic acid [NAA] (8 mg). This was a 17 fold increase compared to field roots (0.47 mg). Conclusion:Therefore C. triloba hairy root cultures are the preferable biological system for anthraquinones production over shoot (0.13 mg) and cell suspension cultures (0.70 mg).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.