The current study aimed to assess the allelopathic effect of Rosmarinus officinalis L. essential oils (EOs) to define the potent effect against weed species, by exploring distinct chemotypes and their main compounds. The EOs from eight accessions were characterized. Their components were identified by gas chromatography, and four chemotypes were defined; C1 (α-pinene), C2 (camphor), C3 (α-pinene/1,8-cineole), and C4 (α-pinene/1,8-cineole/camphor). Four concentrations of the EOs (400, 800, 1200, and 2400 μL/L) and the main compounds of each chemotype were tested in a laboratory assay against Amaranthus retroflexus L. and Lolium perenne L. in pre- and post-germination. The results showed that the EOs significantly affected all the tested parameters (germination, early growth, and physiological and histological parameters of the weeds under study) in a dose, chemotype, and species dependent manner. A. retroflexus was more sensitive than L. perenne at germination level being significantly inhibited at the lowest dose of all the chemotypes. The latter all exhibited significant effects but with a higher potency of C2 (camphor chemotype) and C3 (α-pinene/1,8-cineole chemotype), as well qualitative differences in the induced damage. Our results thus increase knowledge about the role of the monoterpene composition in bio-herbicidal effect, which can help in the development of EO based bio-herbicides.

Green roofs are used increasingly to alleviate peaks of water discharge into the sewage systems in urban areas. Surface runoff from roofs contain pollutants from dry and wet deposition, and green roofs offer a possibility to reduce the amounts of pollutants in the water discharged from roofs by degradation and filtering. These pollutants would otherwise enter wastewater treatments plants and ultimately end up in sewage sludge that is spread on agricultural soils. The most common substrates used in green roofs have limited capacity for filtration and sorption. Also, more sustainable alternatives are sought, due to the high carbon footprint of these materials. Biochar is a carbon-rich material produced by pyrolysis of biomass, and several types of biochar have been described as good sorbents and filter materials. Biochar is also a light and carbon negative material, which may fulfill other desired criteria for new green roof substrates. We here report on an experiment where two types of biochar, produced from olive husks at 450 °C or from forest waste at 850 ° C were mixed with volcanic rock or peat, and tested for retention capacity of phenanthrene and six heavy metals in a column experiment with unsaturated gravimetric water flow lasting for 3 weeks. The results suggest that biochar as a component in green roof substrates perform better than traditional materials, concerning retention of the tested pollutants, and that different types of biochar have different properties in this respect.Electronic supplementary materialThe online version of this article (10.1007/s11356-017-0650-6) contains supplementary material, which is available to authorized users.

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This study used hyperspectral reflectance data to evaluate the crop physiological parameters of sweet maize. Principal component analysis (PCA) was applied to identify the wavelengths that primarily contributed to each selected PC. Correlation analysis and multiple linear regression, with a stepwise algorithm, were used to select the best-performing vegetation indices (VIs) for monitoring the yield and physiological response of sweet maize grown under different water and nitrogen availability. The spectral reflectance measurements of crops were taken during the mid-season stage, for two consecutive growing seasons. The multivariate regression results showed that red-edge group indices, such as CARI (Chlorophyll Absorption Reflectance Index), DD (Double Difference Index), REIP (Red-Edge Inflection Point), and Clred-edge (Chlorophyll Red-Edge) indices were good predictors of yield and physiological parameters, confirming the crucial role of the red-edge spectral region that also emerged through PCA. Moreover, DD, REIP, and Clred-edge VIs were able to discriminate transient temporary stress at the mid-season stage, as well as to separate water and N stress levels. Therefore, hyperspectral reflectance VIs can provide valid information to growers, helping them identify and discriminate between different stress conditions.

Maize is a crucial global commodity, which is used not only for food, but also as an alternative crop in biogas production and as a major energy-supply ingredient in animal diets. However, climate change is jeopardizing current maize production due to its direct impact on weather instability and water availability or its indirect effects on regional climate suitability loss. Hence, new areas for sweet maize cultivation should be considered in the future. Therefore, this study focuses on the possibility of producing maize in a challenging environment in Southern Italy considering rainfed cultivation and two irrigation regimes (full and deficit). The experiment was conducted during two subsequent growing seasons under semi-arid Mediterranean climate conditions. The overall results indicated a significant difference in biomass and yield between irrigated and non-irrigated treatments, and between full and deficit irrigation. Sweet maize cultivated under deficit irrigation gained less biomass than under full irrigation and its development and fruit maturation were delayed. Under deficit irrigation, the plants gave lower yields and a higher percentage of the panicle weight consisted of kernels. Irrigation water productivity was higher for deficit than for full irrigated treatment. These findings indicate the feasibility of sweet maize production in semi-arid areas of Southern Italy using adaptive agricultural strategies including deficit irrigation and controlled water stress. Given the importance of maize production, understanding of maize growth and productivity in a challenging environment may support future agricultural programming and thereby contribute e to mitigation of the direct and indirect effects of climate change.

Soils with low organic matter and modest biological activity exhibit a reduced capacity to produce goods and services. The use of biochar as a soil amendment has been suggested as a way to increase soil productivity thanks to its physical and chemical characteristics. These properties lead to its possible use as a structural support and a habitat for microorganisms. This study investigated the role of biochar, produced from olive mill pomace at the Mediterranean Agronomic Institute of Bari by a slow pyrolysis process, as a carrier of two microbial communities extracted from a fertile soil and a commercial compost. This study aims to highlight the relationship between the physical structure of biochar and the potential microbial community and to evaluate the use of charged biochar as a carrier of microflora. The inoculation of agricultural soil with biochar seems to have no effect on the total aerobic heterotrophic microflora evaluated with the plate count method. Nevertheless, scanning electron microscope (SEM) images show the presence of biofilm, thus suggesting an early stage of colonization. Despite the short time of incubation of microbial charged biochar in the studied soil, the microbial biomass C and N increased, suggesting a beneficial effect of this amendment.

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Polycyclic aromatic hydrocarbons (PAHs) are a large group of compounds composed of two or more aromatic rings. They are extremely toxic pollutants largely produced by anthropogenic activities and characterised by high persistence in the environment. Soils contaminated by PAHs could be depolluted by bioremediation techniques, an effective in-situ procedure which provides the addition of exogenous substrates able to sustain and enhance the autochthonous soil microflora and the allochthon microbial inoculum. Our research aims to study the effects of biochar, produced by slow pyrolysis of olive pomace, as a bio-stimulant of soil microflora or support for the colonisation of the allochthon Trichoderma harzianum, on degradation of pyrene used here as model molecule for the PAH family. Biochar is considered an excellent soil conditioner because of its positive effect on soil physical and chemical properties and its positive interaction with soil microorganisms. Autochthonous microbial growth, T. harzianum growth and microbial pyrene-degradation activity were surveyed in soil samples spiked with 50 ppm of pyrene and incubated for up to 28 days. Pyrene concentration was reduced by ~70% in 28 days in both bioaugmentation and biostimulation tests. Olive mill pomace biochar did not interfere with pyrene bioavailability and did not affect microbial pyrene-degrading activity. The T. harzianum did not display a distinctive ability in degrading pyrene and partially inhibited the endogenous soil microflora.