Small molecules do the job: Somatic cells are reprogrammed into iPS cells upon ectopic expression of Oct4, Sox2, Klf4 and c‐Myc. Application of a cell‐based, high‐throughput chemical screen led to the identification of Src family kinase (SFK) inhibitors as chemical replacements for retroviral Sox2 delivery. These compounds are used to study the mechanisms underlying direct reprogramming and may ultimately help to bring iPS cell technology one step closer to clinical application.
Kleine Moleküle erledigen den Job: Somatische Zellen werden durch ektopische Expression von Oct4, Sox2, Klf4 und c‐Myc in iPS‐Zellen umprogrammiert. Mithilfe eines zellbasierten Hochdurchsatz‐Screenings wurden Inhibitoren der Src‐Kinase (SFK) als „chemischer Ersatz“ für den retroviralen Sox2‐Transport identifiziert. Diese Verbindungen werden genutzt, um die Mechanismen der direkten Umprogrammierung zu untersuchen, und sie könnten letztlich dazu beitragen, die klinische Anwendung von iPS‐Zellen einen Schritt näher zu bringen.
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