Fusarium proliferatum NBRC109045 is a filamentous fungus isolated from Vietnamese forest due to high production of β-glucosidases. Production of the enzyme was studied on varied carbon source based mediums. The highest activity was obtained in medium containing 1% corn stover + 1% wheat bran (3.31 ± 0.14 U/ml). It is interesting to note that glucose (0.69 ± 0.02 U/ml) gave higher activity and just followed by cellobiose among the di- and mono-saccharides, which is generally regarded as a universal repressor of hydrolases. We improved the zymogram method to prove that in response to various carbon sources, F. proliferatum could express various β-glucosidases. One of the β-glucosidases produced by F. proliferatum growing in corn stover + wheat bran based medium was partially purified and proved to have high catalytic ability.
Three new anamorph of Ceramothyrium aquaticum sp. nov., Ceramothyrium exiguum sp. nov., and Ceramothyrium phuquocense sp. nov. are described and illustrated. These fungi were isolated from submerged decaying leaves collected from Phu Quoc National Park, Phu Quoc province, Viet Nam. The phylogeny based on ITS region and D1/D2 of the 28S rDNA gene showed that these fungi nested in the Ceramothyrium. Morphologically, C. aquaticum, C. phuquocense sp. nov. and C. exiguum sp. nov. are characterized; they were different from known anamorph species of Ceramothyrium by having one main axis and two lateral arms with 70 -90, 33.5 -72.5 and 70 -130 µm long main axis, respectively. The table to compare Ceramothyrium anamorph is also given.
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