The agar gel diffusion test (AGDT) was applied to detect Fasciola infection in several regions of Vietnam where equipment for fecal examination such as a microscope is not readily available. A 1% solution of agar dissolved in 10% saline, and the antigen prepared from adult individuals of Fasciola sp. were used for the test. The test was specific for Fasciola infection, which was detected in the sera from about 50% of the cattle and water buffaloes examined. The gel and antigen can be stored in a refrigerator for more than 3 months and used for the test. The results of this study revealed that the agar gel diffusion test could become a useful tool to diagnose fasciolosis in cattle and buffaloes at the Regional Animal Health Centers in Vietnam.Discipline: Animal health Additional key words: liver fluke, rumen fluke, serological test, zoonosis, herd diagnosis JARQ 37 (3), 201 -205
The biological characteristics of isolates of T. evansi collected from buffalo in different provinces in North Vietnam was determined in terms of their sensitivity to drugs currently used in the treatment of trypanosomosis. Five isolates were collected from buffalo, cloned and then tested against Trypamidium, Samorine, Naganol and Veriben. All isolates were sensitive to Naganol and Veriben. An isolate from a buffalo in Ha bac province (Hb1) was the least sensitive with trypamidium at a CD80 > 128 mg/kg, more than 8 times the CD 100 of the remaining isolates (16 mg/kg). An antigen-detection enzyme immunoassay (Ag-ELISA) based on a T. evansi-specific monoclonal antibody was evaluated for its ability to detect infections with T. evansi in buffalo. The sensitivity of the Ag-ELISA was 63% and the specificity 75%. The positive predictive value of this assay was too low to allow identification of individual infected animals on the results of a single test in the districts investigated. For definitive diagnosis, a serial testing protocol was used, where a more specific test, the card agglutination test (CATT) was used initially and any positive samples was then checked by the Ag-ELISA.
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