In this study, three clones of the duckweed genus Lemna, collected from three different regions of Vietnam Binh Thuan Bac Giang and Ben Tre were used to evaluate the growth and development in a culture medium containing different concentrations of As3+, Cd2+, or Pb2+. During 14 days of incubation, the growth rate was determined daily by measuring the surface area of all plants of each sample. Experimental results show that the three Lemna clones could grow in nutrient medium contaminated by heavy metal at a concentration of 0.3-0.5 mg/L for As3+, 0.15-0.3 mg/L for Cd2+, and 0.15 mg/L for Pb2+. At these concentrations, the division rate of the treated clones was several times higher than that of the controls. The highest increase in the surface area was recorded for Lemna BTN (68.47 times higher when cultured in medium supplemented in 0.3 mg/L Pb2+ than under control conditions). The surface area of Lemna BTR cultured in a medium supplemented with 0.3 mg/L As3+ was 54.65 times higher than in medium without arsenic. The obtained results showed that Lemna BGG and Lemna BTR can tolerate arsenic and cadmium pollution while Lemna BTN tolerates lead pollution.
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