Early life stress, including maternal separation, is among one of the main causes of anxiety in adolescents. DNA methyltransferase 3A (Dnmt3a) is a key molecule that regulates DNA methylation and is found to be associated with anxiety-like behavior. It is not clear whether maternal separation affects anxiety levels in mice at different developmental stages, or whether Dnmt3a plays a role in this process. Here, by using open field test to exploring the effect of maternal separation on anxiety-like behavior in mice of different age, it was found that maternal separation could successfully induce anxiety-like behavior in adolescent mice, and which continued through adulthood. By using western blot, we found the levels of Dnmt3a in the hippocampus and cortex have shown different trends in maternal separation mice on P17. Further, by using immunostaining, we have found that the expression levels of Dnmt3a in the cortex and hippocampus were significantly different, and decreased to varying degrees with the age of mice, which being the reason for different trends. Our results provide an experimental basis for further development of anxiety/depression treatment programs more suitable for adolescence.
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