The current study has developed an innovative procedure to generate ex novo fat tissue by culturing adipocytes from human fat tissue mesenchymal stem cells (hFTMSCs) on fibrin gel sheet towards applications in medicine and cosmetology. Fibrin gel has been obtained by combining two components fibrinogen and thrombin collected by human peripheral blood. By this procedure it was possible to generate blocks of fibrin gel containing adipocytes within the gel that show similar features and consistency to human fat tissue mass. Results were assessed by histological staining methods, fluorescent immune-histochemistry staining as well photos by scanning electron microscopy (SEM) to demonstrate the adhesion and growth of cells in the fibrin gel. This result opens a real possibility for future clinical applications in the treatment of reconstructive and regenerative medicine where the use of stem cell may eventually be a unique solution or in the field of aesthetic medicine where autograft fat stem cells may grant for a safer and better outcome with long lasting results.
Platelet-rich plasma (PRP) is a rich source of plateletderived growth factors such as PDGF, TGF and VEGF, making it a potential material in tissue regeneration, especially in regenerative medicine. Due to the limitation of ethical issues for direct research on humans, this study aimed to collect PRP from peripheral blood of the mouse and evaluate its features as platelet concentration, the structure of activated PRP gel, in vitro cytotoxicity and biological characteristics through migration with mouse bone marrow cells. The results showed that the platelet concentration of PRP was five times higher than that in whole blood and PRP has a dense fibrin network after activating by CaCl2 after 10 minutes. PRP is not only safe for cells but also supports bone marrow cells to migrate and close the gap. With these results, PRP can be seen as a material with a considerable potential for wound healing, especially regenerative medicine.
In this work, the angular dependent efficiency recorded by collimated NaI(Tl) detector is determined a quantification of the activity of mono- and multi-energy gamma emitting isotopes positioning in a waste drum. The simulated efficiencies using both MCNP5 and Geant4 are in good agreement with experimental results. Referring to these simulated efficiencies, we recalculated the source activity with the highest deviation of 13%.
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