Simple SummaryLameness is a concern within the dairy industry. Firstly, it has a negative effect on the animal’s welfare, and secondly, has been found to be costly to producers. Ultimately, it can result in having to cull the cow early. Lameness can change the behaviour of cows who may spend more time lying down to relieve the pain in their feet. In this study, we used activity monitors to automatically record the behaviour of non-lame and lame Jersey cattle. Unlike previous studies involving other breeds, the Jersey cows in this study showed no statistically significant differences in the length of time spent lying or standing in relation to lameness status. Lame cows did have significantly shorter average lying bouts compared to non-lame cows. We also recorded what proportion of the herd were lame. The results (38%) were higher than expected in Jersey cattle but may be related to the fact the cows were housed all year round and therefore were not given access to grass. In conclusion, we saw no evidence of an effect of lameness on lying behaviour in this study. AbstractThirty-five lactating Jersey cows were recruited to the study. They were grouped according to locomotion score (LS), where low scores indicate normal gait. LS-1 (n = 12), LS-2 (n = 12) and LS-3 (n = 11) were used. Locomotion scores were balanced for parity and stage of lactation. Lying behaviour was recorded using IceTag™ data loggers attached to the cows for four consecutive days. The study animals remained in the straw based yards with grooved concrete flooring throughout the duration of the study. All data were normally distributed and assessed using a one-way ANOVA with a post hoc Tukey test. There were no statistically significant differences between locomotion score and the time spent lying, active and standing of zero-grazed lactating Jersey dairy cattle housed on straw yards. Lame cows (LS-3) had significantly shorter lying bouts than sound cows (LS-1) (34 min vs. 42 min, respectively). There has been limited research to date measuring the lying behaviour of cattle on straw and into the Jersey breed. The cows had longer than expected standing times and an increased frequency of lying bouts. This may have been attributed to the stocking density in which the cows were kept. We also reported a prevalence of lameness within the herd of 38%.