IRES-EGFP transgenic line constructed here can be used for recovery of proliferation competent cells from different tissue types. STEM CELLS 2007;25:132-138
EMBL accession no. X53333 Triosephosphate isomerase [(TPI), E.C.5.3. 1.11 catalyzes the interconversion of glyceraldehyde 3-phosphate and dihydroxyacetone phosphate, and has an essential role in glycolysis, gluconeogenesis, fatty acid synthesis, and the pentose shunt (1). In mammals, the enzyme consists of two polypeptide chains that are 248 amino acids and identical except for possible post-translational modifications (2). We report here the cDNA sequence and deduced amino acid sequence for murine TPI. The cDNA was isolated from a pcDpolyBNexpression library that was prepared using poly(A)+ RNA from murine P19 cells (3). The cDNA was identified by hybridization to the human counterpart (4, 5). Murine and human TPI cDNA sequences are 87.7% similar within the translated regions and encode proteins that are 94.4% similar. Murine and human 5' and 3' untranslated regions differ in both size and sequence (e.g., the murine 3' untranslated region is 554 bp whereas the human 3' untranslated region is 448 bp), and because of this it is difficult to determine if we have isolated the entire 5' end.
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