A low-cost, innovative and non-invasive colorimetric test, which can be universally used, is proposed to detect pathogenic bacteria via the simple and fast detection of volatile metabolites released by enzymatic hydrolysis. The proof of concept is shown via three sets of experiments studying the release of the p-nitrophenol metabolite in solution in the E. coli cultures containing 4-nitrophenyl-β-d-glucuronide, the trapping efficiency of the gaseous metabolite by various tailored and functionalized xerogels, and the trapping and detection of gaseous p-nitrophenol released by E. coli bacteria.
International audienceA new colorimetric and easy-to-use solid sensor for the detection of indole-producing bacteria is described. The sensor is a nanoporous matrix with tailored pores doped with a reactant, DMACA. The sensor turns rapidly from transparent to green-blue when exposed to metabolic indole coming from bacteria cultures, both in liquid and gas phases. An example of the discrimination between indole-positive (Eschericia coli) and indole-negative bacteria (Hafnia alvei), two important contaminants in food industry is given
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