The orquidáceas are a family of plants with economic and scientific interest given their ornamental diversity and applications in medicine among others. However, many species of orchids are threatened and in danger of extension, due to the transformation of the habitat and the excessive extraction of species with commercial interest. Added to this, its propagation and conservation in vitro, is expensive, due to the biological complexity. The present study reports information on a versatile culture medium, focused on the conservation of germplasm and in-vitro production of orchids. To this end, a formulation was designed based on the modification of the Murashige and Skoog (1962) medium with Morel vitamins, coconut water and activated carbon, using the combination of successful experiences from the reported literature. Capsules of Prosthechea fragrans (Sw.) W.E.Higgins were collected in the coffee axis and sterilized in the laboratory. The culture media were tested for callus induction, proliferation of protocormos type structures, development and maintenance of seedlings. Asymmetric germination in-vitro of collected seeds was achieved, three types of callus were obtained. After 7 months of cultivation, the seedlings reached between 1.1. The sterilization protocol of the capsules was validated as well as the protocol of preparation of medium, sowing and subculture, with respect to the results reported in the literature using conventional culture media. The results show that the modifications made in the MS culture medium, allow to reduce the costs of the culture medium by 10% and make it versatile, allowing the micropropagation and maintenance of seedlings of Prosthechea fragrans (Sw.) W.E.Higgins; present in the coffee axis and the establishment of a germplasm bank for the conservation and production of high quality plant material derived from in vitro culture.
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