Centromeres are the complex structures responsible for the proper segregation of chromosomes during cell division. Structural or functional alterations of the centromere cause aneuploidies and other chromosomal aberrations that can induce cell death with consequences on health and survival of the organism as a whole. Because of their essential function in the cell, centromeres have evolved high flexibility and mechanisms of tolerance to preserve their function following stress, whether it is originating from within or outside the cell. Here, we review the main epigenetic mechanisms of centromeres’ adaptability to preserve their functional stability, with particular reference to neocentromeres and holocentromeres. The centromere position can shift in response to altered chromosome structures, but how and why neocentromeres appear in a given chromosome region are still open questions. Models of neocentromere formation developed during the last few years will be hereby discussed. Moreover, we will discuss the evolutionary significance of diffuse centromeres (holocentromeres) in organisms such as nematodes. Despite the differences in DNA sequences, protein composition and centromere size, all of these diverse centromere structures promote efficient chromosome segregation, balancing genome stability and adaptability, and ensuring faithful genome inheritance at each cellular generation.
Epigenetic regulators play a crucial role in establishing and maintaining gene expression states. To date, the main efforts to study cellular heterogeneity have focused on elucidating the variable nature of the chromatin landscape. Specific chromatin organisation is fundamental for normal organogenesis and developmental homeostasis and can be affected by different environmental factors. The latter can lead to detrimental alterations in gene transcription, as well as pathological conditions such as cancer. Epigenetic marks regulate the transcriptional output of cells. Centromeres are chromosome structures that are epigenetically regulated and are crucial for accurate segregation. The advent of single-cell epigenetic profiling has provided finer analytical resolution, exposing the intrinsic peculiarities of different cells within an apparently homogenous population. In this review, we discuss recent advances in methodologies applied to epigenetics, such as CUT&RUN and CUT&TAG. Then, we compare standard and emerging single-cell techniques and their relevance for investigating human diseases. Finally, we describe emerging methodologies that investigate centromeric chromatin specification and neocentromere formation.
This study employs the circular restricted three-body problem (CR3BP) as the dynamical framework, for the purpose of investigating low-thrust orbit dynamics in the Earth–Moon system. First, the effect of low thrust on some dynamical structures that exist in the CR3BP is analyzed. Low-thrust capture and escape dynamics in the proximity of the Moon is investigated for preliminary mission analysis. Then, low-thrust periodic orbits—with potential practical application—are detected. To do this, the theorem of mirror trajectories, proven 6 decades ago, is extended to low-thrust trajectories. This represents the theoretical premise for the definition and use of a numerical search methodology based on modified Poincaré maps. This approach leads to identifying several low-thrust periodic orbits in the Earth–Moon system that are infeasible if only unpowered paths are considered. Two possible applications of low-thrust periodic orbits are described: (a) cycling transfer trajectories that connect Earth and Moon continuously, and (b) non-Keplerian periodic paths about the Moon, with potential use as operational orbits for satellite constellations.
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