Sphingosine kinase 1 (SphK1) and SphK2 are ubiquitous enzymes that generate sphingosine-1-phosphate (S1P), a ligand for a family of G protein-coupled receptors (S1PR1-S1PR5) with important functions in the vascular and immune systems. Here we explore the role of these kinases and receptors in recovery from anaphylaxis in mice. We found that Sphk2 -/-mice had a rapid recovery from anaphylaxis. In contrast, Sphk1 -/-mice showed poor recovery from anaphylaxis and delayed histamine clearance. Injection of S1P into Sphk1 -/-mice increased histamine clearance and promoted recovery from anaphylaxis. Adoptive cell transfer experiments demonstrated that SphK1 activity was required in both the hematopoietic and nonhematopoietic compartments for recovery from anaphylaxis. Mice lacking the S1P receptor S1PR2 also showed a delay in plasma histamine clearance and a poor recovery from anaphylaxis. However, S1P did not promote the recovery of S1pr2 -/-mice from anaphylaxis, whereas S1pr2 +/-mice showed partial recovery. Unlike Sphk2 -/-mice, Sphk1 -/-and S1pr2 -/-mice had severe hypotension during anaphylaxis. Thus, SphK1-produced S1P regulates blood pressure, histamine clearance, and recovery from anaphylaxis in a manner that involves S1PR2. This suggests that specific S1PR2 agonists may serve to counteract the vasodilation associated with anaphylactic shock.
Introducción: el apego materno es el conjunto de conductas y vínculos que la madre establece en torno al recién nacido. El ingreso en la Unidad de Cuidados Intensivos Neonatales supone una barrera en el establecimiento de estos primeros lazos.<br /><br />Objetivo: el principal objetivo del presente estudio es comparar el grado de apego valorando dicha separación, así como identificar aquellas variables que podrían influir en su establecimiento.<br /><br />Material y métodos: un total de 82 madres, divididas en dos grupos (O-U), participaron en este estudio ex postfacto comparativo causal mediante la cumplimentación del cuestionario Maternal Attachment Inventory, la entrevista y la revisión de la historia clínica.<br /><br />Resultados: se encontraron diferencias estadísticamente significativas en la puntuación MAI entre madres del Grupo Obstetricia (99’67) y del Grupo UCIN (91’64), del mismo modo se señaló la edad gestacional y la alimentación como factores influyentes. <br /><br />Discusión y conclusión: la separación tras el ingreso en la Unidad de Cuidados Intensivos Neonatales dificulta el establecimiento de los primeros vínculos maternos. Es necesario valorar los diferentes factores que influyen en esta situación y realizar futuras intervenciones para mejorar la relación materno-filial. <br /><br />
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